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Fluorogenic mercury ion sensor based on pyrene-amino mercapto thiadiazole unit
Journal of Hazardous Materials ( IF 12.2 ) Pub Date : 2017-09-15 , DOI: 10.1016/j.jhazmat.2017.09.028
B. Kirthika Rani , S. Abraham John

A highly selective and sensitive determination of Hg2+ in water samples with bioimaging applications in living cells using a fluorogenic pyrene-amino mercapto thiadiazole (PYAMT) probe is described. The probe PYAMT exhibited three absorption peaks at 333, 348 and 394 nm and emission maxima at 378, 388 and 397 nm (λex = 348 nm). It showed significant fluorescent quenching (96%) with I/I0 = 0.051 upon the addition of 2.5 μM Hg2+ ion in CH3CN:H2O (1:1, v/v; pH 7.2), whereas its fluorescence remained unaltered in the presence of other metal ions. The quenching phenomenon is attributed to the heavy atom effect of Hg2+ ion followed by electron transfer. The fluorescence intensity decreased linearly against a wide range from 100 nM to 2.5 μM Hg2+ (R2 = 0.9937) with a limit of detection as low as 0.35 nM (S/N = 3). The binding stoichiometry ratio of PYAMT-Hg2+ is proved to be 1:1 by fluorescence and DFT measurements. The sensor possesses high association constant with Hg2+ ion in the order of 9.08 × 105 M−1 and it is also capable of reversibly detecting cysteine with OFF-ON mechanism. Finally, the proposed method is successfully applied to selectively detect Hg2+ ion in real water samples and bioimaging studies in live cells.

中文翻译:

基于pyr-氨基巯基噻二唑单元的荧光汞离子传感器

描述了使用荧光pyr-氨基巯基噻二唑(PYAMT)探针对活细胞进行生物成像应用的水样品中Hg 2+的高选择性和灵敏测定方法。探针PYAMT在333、348和394 nm处表现出三个吸收峰,在378、388和397 nm处表现出发射最大值(λex  = 348 nm)。在CH 3 CN:H 2 O(1:1,v / v; pH 7.2)中 加入2.5μMHg 2+离子后,它显示出显着的荧光猝灭(96%),I / I 0 = 0.051在其他金属离子的存在下保持不变。猝灭现象归因于Hg 2+的重原子效应离子,然后进行电子转移。荧光强度在从100 nM到 2.5μMHg 2+的宽范围内线性下降(R 2 = 0.9937),检出限低至0.35 nM(S / N = 3)。通过荧光和DFT测量证明,PYAMT-Hg 2+的结合化学计量比为1:1。该传感器与Hg 2+离子的结合常数高,为9.08×10 5  M -1,并且还可以通过OFF-ON机制可逆地检测半胱氨酸。最终,该方法成功应用于选择性检测真实水样中的Hg 2+离子和活细胞的生物成像研究。
更新日期:2017-09-15
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