Fluralaner is a novel isoxazoline insecticide which shows high insecticidal activity against parasitic, sanitary and agricultural pests, but there is little information about the effect of fluralaner on non-target organisms. This study reports the acute toxicity, bioconcentration, elimination and antioxidant response of fluralaner in zebrafish. All LC₅₀ values of fluralaner to zebrafish were higher than 10 mg L⁻¹ at 24, 48, 72 and 96 h. To study the bioconcentration and elimination, the zebrafish were exposed to sub-lethal concentrations of fluralaner (2.00 and 0.20 mg L⁻¹) for 15 d and then held 6 d in clean water. The results showed medium BCF of fluralaner with values of 12.06 (48 h) and 21.34 (144 h) after exposure to 2.00 and 0.20 mg L⁻¹ fluralaner, respectively. In the elimination process, a concentration of only 0.113 mg kg⁻¹ was found in zebrafish on the 6th day after removal to clean water. After exposure in 2.00 mg L⁻¹ fluralaner, the enzyme activities of SOD, CAT, and GST, GSH-PX, CarE and content of MDA were measured. Only CAT and CarE activities were significantly regulated and the others stayed at a stable level compared to the control group. Meanwhile, transcriptional expression of CYP1C2, CYP1D1, CYP11A were significantly down-regulated at 12 h exposed to 2.00 mg L⁻¹ of fluralaner. Except CYP1D1, others CYPs were up-regulated at different time during exposure periods.

Fluralaner and its formulated product (BRAVECTO^®) are of low toxicity to zebrafish and are rapidly concentrated in zebrafish and eliminated after exposure in clean water. Antioxidant defense and metabolic systems were involved in the fluralaner-induced toxicity. Among them, the activities of CAT and CarE, and most mRNA expression level of CYPs showed fast response to the sub-lethal concentration of fluralaner, which could be used as a biomarker relevant to the toxicity.

Fluralaner是一种新型的异恶唑啉杀虫剂，对寄生虫，卫生和农业害虫显示出很高的杀虫活性，但是关于fluralaner对非目标生物的影响的信息很少。这项研究报告了斑马鱼中fluralaner的急性毒性，生物浓度，消除作用和抗氧化反应。在24、48、72和96 h时，氟尿烷到斑马鱼的所有LC ₅₀值均高于10 mg L ^-1。为了研究生物富集和消除，将斑马鱼暴露于亚致死浓度的氟尿烷（2.00和0.20 mg L ^-1）中15 d，然后在净水中放置6 d。结果显示，暴露于2.00和0.20 mg L ^-1后，氟尿烷的中等BCF值分别为12.06（48 h）和21.34（144 h）fluralaner，分别。在消除过程中，在移至干净水后第6天，斑马鱼中的浓度仅为0.113 mg kg ^-1。暴露于2.00 mg L ^-1氟尿烷中后，测量SOD，CAT和GST，GSH-PX，Care的酶活性和MDA含量。与对照组相比，只有CAT和CarE活性受到显着调节，其他的则保持稳定水平。同时，CYP1C2，CYP1D1，CYP11A的转录表达在暴露于2.00 mg L ^-1的氟尿烷中12 h时显着下调。除CYP1D1外，其他CYP在暴露期间的不同时间上调。

Fluralaner及其配制的产品（BRAVECTO ^®）是低毒性的斑马鱼和迅速集中在斑马鱼和在干净的水暴露后消除。抗氧化剂防御和代谢系统参与了氟尿素诱导的毒性。其中，CAT和CarE的活性以及CYPs的大多数mRNA表达水平显示出对氟尿嘧啶亚致死浓度的快速反应，可以用作与毒性相关的生物标志物。