Fabricating membrane adsorbers with high adsorption capacity and appreciable throughput for the separation and purification of protein products is challenging in biomedical and pharmaceutical industries. Herein, we report the synthesis of a novel membrane adsorber by functionalizing a nylon microfiltration membrane with alginate dialdehyde (ADA) followed by sulphonic addition, without any solvent usage, and its successful application in the purification of lysozyme. Taking advantage of abundant dual cation exchange (CEX) groups on sulphonic-ADA (S-ADA) ligands, this novel S-ADA-nylon membrane adsorber showed an unprecedented static binding capicity of 286 mg/mL for lysozyme adsorption. Meanwhile, the prepared membrane adsorber could be easily regenerated (complete protein elution) under mild conditions and be reused at least for five times. Featured with a unique selectivity, the S-ADA-nylon membrane also captured lysozyme from chicken egg white solution with a high purity (100%) and a high recovery of 98%. The purified lysozyme showed similar specific activity as commercial product. The present work provides a facile, green and low-cost approach for the preparation of high-performance membrane adsorbers, which has a great potential in protein production.

在生物医学和制药工业中，制造具有高吸附能力和可观通量以分离和纯化蛋白质产物的膜吸附器是具有挑战性的。在这里，我们报告了新型的膜吸附剂的合成，该方法通过使用藻酸盐二醛（ADA）对尼龙微滤膜进行功能化，然后在不使用任何溶剂的情况下进行磺酸加成而实现，并将其成功应用于溶菌酶的纯化。该新型S-ADA-尼龙膜吸附剂利用磺酸-ADA（S-ADA）配体上丰富的双阳离子交换（CEX）基团，对溶菌酶的吸附显示出前所未有的286 mg / mL的静态结合能力。同时，所制备的膜吸附剂可以在温和条件下容易地再生（完全蛋白洗脱），并且至少可以重复使用五次。S-ADA尼龙膜具有独特的选择性，还可以从鸡肉蛋白溶液中捕获溶菌酶，纯度高（100％），回收率高达98％。纯化的溶菌酶显示出与商品相似的比活性。本工作为制备高性能膜吸附剂提供了一种简便，绿色，低成本的方法，该方法在蛋白质生产中具有巨大的潜力。