The ultrathin NiFe-layered double hydroxide nanosheets (NiFe-LDHNS) were prepared by exfoliating bulk LDH in l-asparagine aqueous medium. The transmission electron microscopy and atomic force microscopy results reveal that NiFe-LDHNS have about 200–300 nm lateral size and less than 2 nm thickness with the broken edges rather than the original hexagon of hydrotalcite. The peroxidase-like activity of the 2D NiFe-LDHNS was investigated by using oxidation of the peroxidase substrate 3,3′,5,5′-tetramethylbenzidine in presence of H₂O₂. The 2D NiFe-LDHNS exhibited the superior enzyme mimic activity to the bulk NiFe-LDH, which was attributed to the more exposed active sites and more accessible inner surface of single or several layers. Thus, a simple and sensitive non-enzyme biosensor was developed for colorimetric detection of H₂O₂ and glucose. The proposed method was successfully applied to glucose detection in real samples with satisfactory results.

通过在1-天冬酰胺水介质中剥离大量的LDH，制备超薄的NiFe层状双氢氧化物纳米片（NiFe-LDHNS）。透射电子显微镜和原子力显微镜的结果表明，NiFe-LDHNS的侧向尺寸约为200-300 nm，厚度小于2 nm，且边缘破裂，而不是水滑石的原始六边形。通过在H ₂ O ₂存在下使用过氧化物酶底物3,3'，5,5'-四甲基联苯胺进行氧化，研究了二维NiFe-LDHNS的过氧化物酶活性。。二维NiFe-LDHNS表现出比块状NiFe-LDH更好的酶模拟活性，这归因于更多的活性位点和单层或多层的内表面。因此，开发了一种简单而灵敏的非酶生物传感器，用于比色检测H ₂ O ₂和葡萄糖。该方法成功应用于实际样品中的葡萄糖检测，结果令人满意。