A new kind of designed water-compatible molecularly imprinted conductive nanoparticles was prepared by combining macromolecular self-assembly with molecularly imprinting technique. An amphiphilic random copolymer poly(DMA-co-EHA-co-HEA-co-St) containing imino groups and photocrosslinking units was synthesized through free radical copolymerization, which could self-assemble with template molecules (glucose) in aqueous solution to establish nanoparticles embedded with glucose. Meanwhile, AuCl₄⁻, as precursor, was added to generate Au NPs in situ because of reducibility of protonated imino groups, leading to the formation of molecularly imprinted polymeric nanoparticles decorated with Au NPs ([email protected]). By simply adjusting the pH value of the assembly solution, [email protected] with different loading amount of Au NPs could be obtained. Electrodeposition was employed to fabricate a robust integration of MIP film with a transducer, then the film was photo-crosslinked by UV irradiation. After extraction of the template molecules, an MIP sensor modified with Au NPs ([email protected] sensor) was successfully constructed. The presence of Au NPs greatly improved the conductivity of [email protected] film and allowed direct electrical connection between the recognition cavities and the transducer, which enhanced the sensitivity of the sensor for templates detection. Effect of Au NPs amount on the performance of the [email protected] sensor was also evaluated. The obtained [email protected] sensor demonstrated acceptable selectivity and wider linear ranges (two linear ranges from 10⁻¹⁰ to 10⁻⁸ mol L⁻¹ and 10⁻⁸ to 10⁰ mol L⁻¹ with a detection limit of 3×10⁻¹² mol L⁻¹) for glucose detection than MIP sensor without decoration of Au NPs.

高分子自组装技术与分子印迹技术相结合，制备了一种新型设计的水相容性分子印迹导电纳米颗粒。两亲性无规共聚物聚（DMA-共-EHA-共-HEA-共-St）含有亚氨基和光交联单元是通过自由基共聚合成，这可能在水溶液中以建立纳米颗粒的模板分子（葡萄糖）自组装嵌入葡萄糖。同时，AUCL ₄ ^- ，作为前体，加入到生成的金纳米粒子在原位由于质子化亚氨基的可还原性，导致形成装饰有金纳米颗粒的分子印迹聚合物纳米颗粒（[受电子邮件保护]）。通过简单地调节组装溶液的pH值，可以获得具有不同负载量的Au NP的[电子邮件保护]。用电沉积法将MIP膜与换能器牢固地结合在一起，然后通过UV辐照使膜光致交联。提取模板分子后，成功构建了用Au NPs修饰的MIP传感器（[电子邮件保护]传感器）。Au NPs的存在极大地提高了[受电子邮件保护的]薄膜的电导率，并允许识别腔和换能器之间直接电连接，从而提高了用于模板检测的传感器的灵敏度。还评估了Au NPs量对[受电子邮件保护的]传感器性能的影响。获得的[受电子邮件保护的]传感器显示出可接受的选择性和更宽的线性范围（从10开始有两个线性范围^-10至10 ^-8 摩尔大号^-1和10 ^-8〜10 ⁰ 摩尔大号^-1以3×10的检测极限^-12 摩尔大号^-1葡萄糖检测比MIP传感器），而不金纳米粒子的装饰。