A three-step strategy was developed for systematic characterization of curcuminoids in turmeric. Based on UHPLC-QTOF-MS/MS analysis, 89 curcuminoids including 16 novel ones were identified in the turmeric samples using this approach. During the identification process, false positive results were excluded by combining the positive and negative ESI-MS/MS analyses. Moreover, the characterization of the keto and enol forms of type A, B and C curcuminoids was first discussed and they were clearly distinguished using negative ESI-MS/MS method with UV spectra analyses. The structures of detected curcuminoids were identified and rationalized in both ion modes. Additionally, the fragmentation behaviors of the 15 types of curcuminoids were clearly illustrated in this work, which will be helpful for detection and identification of corresponding trace curcuminoids in complex turmeric samples using UHPLC-QTOF-MS/MS methods.

针对姜黄中姜黄素的系统表征，开发了一种三步策略。基于UHPLC-QTOF-MS / MS分析，使用此方法在姜黄样品中鉴定出89种姜黄素，包括16种新姜黄素。在鉴定过程中，通过结合阳性和阴性ESI-MS / MS分析排除了假阳性结果。此外，首先讨论了A，B和C型姜黄素的酮和烯醇形式的表征，并使用带有紫外光谱分析的阴性ESI-MS / MS方法对其进行了清晰区分。在两种离子模式下，都鉴定并合理化了检测到的姜黄素的结构。此外，这项工作清楚地说明了15种姜黄素的碎裂行为，