In this study, a particular pectic polysaccharide (FPLP) was extracted and purified from the fruits of Ficus pumila Linn. through boiling water extraction, alcohol precipitation, diethylaminoethyl-Sepharose Fast Flow chromatography and Superdex™ G-75 gel filtration chromatography. Analysis of high-performance gel permeation chromatography, FTIR, GC–MS, methylation and 1D/2D NMR spectroscopy revealed that FPLP (Mw: 34.69 kDa) is a linear (1,4)-α-d-galacturonic acid binding 1.30% branched chain hexenuronic acid with 23.34% methyl esterification. Treatment with FPLP ameliorated hyperglycaemia in association with an improvement in hepatic glycogen metabolism in C57BL/KsJ db/db mice. The activation of IRS-1/PI3K/Akt/GSK3β/GS insulin signalling pathway and AMPK/GSK3β/GS signalling pathway and the regulation of glucokinase, phosphoenolpyruvate carboxykinase and glucose-6-phosphatase expressions involved in hepatic glycogenesis and glycogenolysis were considered the therapeutic mechanisms of FPLP. These results provide a new insight for investigating the effects of pectic polysaccharides on blood glucose control and suggest that FPLP is a promising nutraceutical for treatment of T2DM.

中文翻译：

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榕果果实中的果糖醛酸与果糖醛酸对C57BL / KsJ db / db小鼠的降血糖作用及其机制

在这项研究中，从无花果榕的果实中提取并纯化了一种特定的果胶多糖（FPLP）。通过沸水萃取，酒精沉淀，二乙氨基乙基-琼脂糖快速流动色谱和Superdex™G-75凝胶过滤色谱。高效凝胶渗透色谱，FTIR，GC-MS，甲基化和1D / 2D NMR光谱分析表明，FPLP（Mw：34.69 kDa）是线性（1,4）-α- d-半乳糖醛酸结合1.30％的支链己醛糖醛酸，甲基酯化率为23.34％。FPLP治疗可改善C57BL / KsJ db / db小鼠肝糖原代谢并改善其高血糖症。IRS-1 / PI3K / Akt /GSK3β/ GS胰岛素信号通路和AMPK /GSK3β/ GS信号通路的激活以及涉及肝糖原和糖原分解的葡萄糖激酶，磷酸烯醇丙酮酸羧激酶和葡萄糖-6-磷酸酶表达的调节被认为是治疗性的FPLP的机制。这些结果为研究果胶多糖对血糖控制的影响提供了新的见识，并表明FPLP是治疗T2DM的有前途的保健食品。