Screen-Printed Polyaniline-Based Electrodes for the Real-Time Monitoring of Loop-Mediated Isothermal Amplification Reactions,Analytical Chemistry

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Screen-Printed Polyaniline-Based Electrodes for the Real-Time Monitoring of Loop-Mediated Isothermal Amplification Reactions
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-09-14 00:00:00 , DOI: 10.1021/acs.analchem.7b02394
David Gosselin _{1,

2,

3} , Maxime Gougis _{1,

2} , Mélissa Baque _{1,

2} , Fabrice P. Navarro _{1,

2} , Mohamed N. Belgacem ₃ , Didier Chaussy ₃ , Anne-Gaëlle Bourdat _{1,

2} , Pascal Mailley _{1,

2} , Jean Berthier _{1,

2}

Affiliation

Nucleic acid amplification testing is a very powerful method to perform efficient and early diagnostics. However, the integration of a DNA amplification reaction with its associated detection in a low-cost, portable, and autonomous device remains challenging. Addressing this challenge, the use of screen-printed electrochemical sensor is reported. To achieve the detection of the DNA amplification reaction, a real-time monitoring of the hydronium ions concentration, a byproduct of this reaction, is performed. Such measurements are done by potentiometry using polyaniline (PAni)-based working electrodes and silver/silver chloride reference electrodes. The developed potentiometric sensor is shown to enable the real-time monitoring of a loop-mediated isothermal amplification (LAMP) reaction with an initial number of DNA strands as low as 10 copies. In addition, the performance of this PAni-based sensor is compared to fluorescence measurements, and it is shown that similar results are obtained for both methods.

中文翻译：

丝网印刷的聚苯胺基电极用于环介导的等温扩增反应的实时监测

核酸扩增测试是执行有效的早期诊断的非常有效的方法。然而，在低成本，便携式和自主装置中将DNA扩增反应与其相关检测结合起来仍然是挑战。为了解决这一挑战，据报道使用了丝网印刷的电化学传感器。为了实现DNA扩增反应的检测，实时监测该反应的副产物水合氢离子浓度。使用基于聚苯胺（PAni）的工作电极和银/氯化银参比电极通过电位计完成此类测量。显示出开发的电位传感器可以实时监测回路介导的等温扩增（LAMP）反应，初始DNA链数可低至10个拷贝。

更新日期：2017-09-15

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