Design of Cyclic Peptide Based Glucose Receptors and Their Application in Glucose Sensing,Analytical Chemistry

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Design of Cyclic Peptide Based Glucose Receptors and Their Application in Glucose Sensing
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-09-14 00:00:00 , DOI: 10.1021/acs.analchem.7b02430
Chao Li ₁ , Xin Chen ₁ , Fuyuan Zhang ₁ , Xingxing He ₁ , Guozhen Fang ₁ , Jifeng Liu ₁ , Shuo Wang ₁

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Glucose assay is of great scientific significance in clinical diagnostics and bioprocess monitoring, and to design a new glucose receptor is necessary for the development of more sensitive, selective, and robust glucose detection techniques. Herein, a series of cyclic peptide (CP) glucose receptors were designed to mimic the binding sites of glucose binding protein (GBP), and CPs’ sequence contained amino acid sites Asp, Asn, His, Asp, and Arg, which constituted the first layer interactions of GBP. The properties of these CPs used as a glucose receptor or substitute for the GBP were studied by using a quartz crystal microbalance (QCM) technique. It was found that CPs can form a self-assembled monolayer at the Au quartz electrode surface, and the monolayer’s properties were characterized by using cyclic voltammetry, electrochemical impedance spectroscopy, and atomic force microscopy. The CPs’ binding affinity to saccharide (i.e., galactose, fructose, lactose, sucrose, and maltose) was investigated, and the CPs’ sensitivity and selectivity toward glucose were found to be dependent upon the configuration,i.e., the amino acids sequence of the CPs. The cyclic unit with a cyclo[-CNDNHCRDNDC-] sequence gave the highest selectivity and sensitivity for glucose sensing. This work suggests that a synthetic peptide bearing a particular functional sequence could be applied for developing a new generation of glucose receptors and would find huge application in biological, life science, and clinical diagnostics fields.

中文翻译：

基于环肽的葡萄糖受体的设计及其在葡萄糖传感中的应用

葡萄糖测定在临床诊断和生物过程监测中具有重要的科学意义，设计新的葡萄糖受体对于开发更灵敏，选择性和耐用的葡萄糖检测技术是必需的。在本文中，设计了一系列环肽（CP）葡萄糖受体以模拟葡萄糖结合蛋白（GBP）的结合位点，并且CP的序列包含氨基酸位点Asp，Asn，His，Asp和Arg，这构成了第一个GBP的图层交互。通过使用石英晶体微量天平（QCM）技术研究了用作葡萄糖受体或GBP替代物的这些CP的性能。发现CPs可以在Au石英电极表面形成自组装单分子层，并通过循环伏安法表征了单分子层的性能，电化学阻抗谱和原子力显微镜。研究了CP对糖（即半乳糖，果糖，乳糖，蔗糖和麦芽糖）的结合亲和力，发现CP对葡萄糖的敏感性和选择性取决于其构型，即氨基酸的氨基酸序列。 CP。带环[-C的循环单元NDNH C RDND C-]序列为葡萄糖传感提供了最高的选择性和灵敏度。这项工作表明，带有特定功能序列的合成肽可用于开发新一代的葡萄糖受体，并将在生物学，生命科学和临床诊断领域中得到广泛应用。

更新日期：2017-09-15

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