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Lysosomal-Targeted Two-Photon Fluorescent Probe to Sense Hypochlorous Acid in Live Cells
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-09-14 00:00:00 , DOI: 10.1021/acs.analchem.7b02361 Beibei Zhang 1 , Xiaopeng Yang 1 , Rui Zhang 2 , Yao Liu 1 , Xueling Ren 2 , Ming Xian 3 , Yong Ye 1, 4 , Yufen Zhao 1, 4
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-09-14 00:00:00 , DOI: 10.1021/acs.analchem.7b02361 Beibei Zhang 1 , Xiaopeng Yang 1 , Rui Zhang 2 , Yao Liu 1 , Xueling Ren 2 , Ming Xian 3 , Yong Ye 1, 4 , Yufen Zhao 1, 4
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A two-photon reversible fluorescent probe L1 was designed and synthesized. The fluorescence intensity of the probe solution was strong, while the fluorescence of the solution was obviously quenched and the color of the solution was changed after the addition of hypochlorous acid, indicating this is “naked-eye sensor” for the detection of HClO. The probe showed great selectivity for hypochlorous acid over other reactive oxygen species (ROS) and anions. Fluorescence titration experiments showed that the probe has a low detection limit of 0.674 μM. Because of a morpholine group introduced to the naphathalimide framework, probe L1 was successfully applied to detect intracellular HClO in lysosome.
中文翻译：
溶酶体靶向的双光子荧光探针可检测活细胞中的次氯酸
设计并合成了一个两光子可逆荧光探针L1。加入次氯酸后，探针溶液的荧光强度很强，溶液的荧光明显淬灭，溶液的颜色发生了变化，这是用于检测HClO的“裸眼传感器”。该探针对次氯酸的选择性高于其他活性氧（ROS）和阴离子。荧光滴定实验表明该探针的检测限很低，为0.674μM。由于将吗啉基引入到萘甲酰亚胺骨架中，探针L1成功地用于检测溶酶体中的细胞内HClO。
更新日期：2017-09-14
中文翻译：
溶酶体靶向的双光子荧光探针可检测活细胞中的次氯酸
设计并合成了一个两光子可逆荧光探针L1。加入次氯酸后，探针溶液的荧光强度很强，溶液的荧光明显淬灭，溶液的颜色发生了变化，这是用于检测HClO的“裸眼传感器”。该探针对次氯酸的选择性高于其他活性氧（ROS）和阴离子。荧光滴定实验表明该探针的检测限很低，为0.674μM。由于将吗啉基引入到萘甲酰亚胺骨架中，探针L1成功地用于检测溶酶体中的细胞内HClO。
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