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Design of Multifunctional Nanostructure for Ultrafast Extraction and Purification of Aflatoxins in Foodstuffs
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-09-13 00:00:00 , DOI: 10.1021/acs.analchem.7b02777 Jie Xie 1 , Haiyang Jiang 1 , Jianzhong Shen 1 , Tao Peng 1 , Jianyi Wang 1 , Kai Yao 1 , Shujuan Sun 1 , Bing Shao 1 , Junwang Tang 2
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-09-13 00:00:00 , DOI: 10.1021/acs.analchem.7b02777 Jie Xie 1 , Haiyang Jiang 1 , Jianzhong Shen 1 , Tao Peng 1 , Jianyi Wang 1 , Kai Yao 1 , Shujuan Sun 1 , Bing Shao 1 , Junwang Tang 2
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Aflatoxins (AFs) are a class of carcinogens, associated with liver cancers, that exist in foodstuffs. There are extremely low maximum limits of AFs in foodstuffs (0.025–20 μg·kg–1). Quick and sensitive detection of such low concentration of AFs in foodstuffs is dominated by the efficiency and selectivity of the AF enrichment process, which is extremely challenging although substantial efforts have been made in recent decades. Here we design and synthesize a multilayer nanoarchitecture composed of a broad-spectrum aflatoxin monoclonal antibody shell, chitosan middle layer, and magnetic bead core (denoted AF-mAb/CTS/Fe3O4). The efficiency of AF-mAb/CTS/Fe3O4 in extracting AFs has been found to be more than 60 times higher than both conventional immunoaffinity chromatography and solid-phase extraction. Furthermore, the nanocomposite displays excellent selectivity and good reusability as well as outstanding efficiency. When coupled to ultraperformance liquid chromatography–tandem quadrupole mass spectrometry, this new nanoarchitecture enables us to probe six AFs at concentrations as low as 0.003 μg·kg–1 in foodstuffs with free matrix effects, which is nearly 10 times smaller than the regulated maximum tolerated does. It is believed that the new nanoarchitecture will provide an efficient and fast pathway to detect AFs in foodstuffs to protect human being from some critical liver cancers.
中文翻译：
食品中黄曲霉毒素超快速提取纯化的多功能纳米结构设计
黄曲霉毒素（AFs）是一类与肝癌相关的致癌物质，存在于食品中。食品中AF的最大限量极低（0.025–20μg·kg –1）。食品中如此低浓度的AF的快速，灵敏检测主要由AF富集过程的效率和选择性决定，尽管近几十年来已经做出了巨大努力，但是这非常具有挑战性。在这里，我们设计并合成了多层纳米结构，该结构由广谱黄曲霉毒素单克隆抗体壳，壳聚糖中间层和磁珠芯（表示为AF-mAb / CTS / Fe 3 O 4）组成。AF-mAb / CTS / Fe 3 O 4的效率已经发现，AF的提取比常规免疫亲和色谱法和固相提取法高60倍以上。此外，纳米复合材料显示出优异的选择性和良好的可重复使用性以及出色的效率。当与超高效液相色谱-串联四极杆质谱联用时，这种新的纳米结构使我们能够以低至0.003μg·kg –1的浓度检测食品中具有游离基质效应的六种AF，几乎是规定的最大耐受量的十倍做。人们相信，新的纳米结构将提供检测食品中AF的有效且快速的途径，以保护人类免受某些严重的肝癌的侵害。
更新日期：2017-09-14
中文翻译：
食品中黄曲霉毒素超快速提取纯化的多功能纳米结构设计
黄曲霉毒素（AFs）是一类与肝癌相关的致癌物质，存在于食品中。食品中AF的最大限量极低（0.025–20μg·kg –1）。食品中如此低浓度的AF的快速，灵敏检测主要由AF富集过程的效率和选择性决定，尽管近几十年来已经做出了巨大努力，但是这非常具有挑战性。在这里，我们设计并合成了多层纳米结构，该结构由广谱黄曲霉毒素单克隆抗体壳，壳聚糖中间层和磁珠芯（表示为AF-mAb / CTS / Fe 3 O 4）组成。AF-mAb / CTS / Fe 3 O 4的效率已经发现，AF的提取比常规免疫亲和色谱法和固相提取法高60倍以上。此外，纳米复合材料显示出优异的选择性和良好的可重复使用性以及出色的效率。当与超高效液相色谱-串联四极杆质谱联用时，这种新的纳米结构使我们能够以低至0.003μg·kg –1的浓度检测食品中具有游离基质效应的六种AF，几乎是规定的最大耐受量的十倍做。人们相信，新的纳米结构将提供检测食品中AF的有效且快速的途径，以保护人类免受某些严重的肝癌的侵害。
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