In this study, we report the development of a high sensitivity assay for the detection of cardiac troponin I using electrical double layer gated high field AlGaN/GaN HEMT biosensor. The unique gating mechanism overcomes the drawback of charge screening seen in traditional FET based biosensors, allowing detection of target proteins in physiological solutions without sample processing steps. Troponin I specific antibody and aptamer are used as receptors. The tests carried out using purified protein solution and clinical serum samples depict high sensitivity, specificity and wide dynamic range (0.006–148 ng/mL). No additional wash or sample pre-treatment steps are required, which greatly simplifies the biosensor system. The miniaturized HEMT chip is packaged in a polymer substrate and easily integrated with a portable measurement unit, to carry out quantitative troponin I detection in serum samples with < 2 µl sample volume in 5 min. The integrated prototype biosensor unit demonstrates the potential of the method as a rapid, inexpensive, high sensitivity CVD biomarker assay. The highly simplified protocols and enhanced sensor performance make our biosensor an ideal choice for point of care diagnostics and personal healthcare systems.

在这项研究中，我们报告了使用双层电控高场AlGaN / GaN HEMT生物传感器检测心肌肌钙蛋白I的高灵敏度测定方法的开发。独特的门控机制克服了传统基于FET的生物传感器中电荷筛选的缺点，无需样品处理步骤即可检测生理溶液中的目标蛋白。肌钙蛋白I特异性抗体和适体用作受体。使用纯化的蛋白质溶液和临床血清样品进行的测试显示出高灵敏度，特异性和宽动态范围（0.006–148 ng / mL）。无需额外的清洗或样品预处理步骤，从而大大简化了生物传感器系统。小型化的HEMT芯片封装在聚合物基板中，可轻松与便携式测量单元集成在一起，在5分钟内对样品体积小于2 µl的血清样品中的肌钙蛋白I进行定量检测。集成的原型生物传感器单元展示了该方法作为一种快速，廉价，高灵敏度的CVD生物标志物检测方法的潜力。高度简化的协议和增强的传感器性能使我们的生物传感器成为即时诊断和个人医疗保健系统的理想选择。