Electrochemiluminescent (ECL) assay has gradually drawn increasing interest in the biomedical analysis. This paper proposed a new methodology for ultrasensitive and facile detection of Alzheimer's disease marker β-amyloid (Aβ) by fabricating a sandwich-type ECL sensing platform. Herein, electrochemiluminescence resonance energy transfer (ECL-RET) was employed to determine Aβ concentration, which can be attributed to the quenching effect from RET between Ru(bpy)₃²⁺ and gold nanorods (GNRs) acting as ECL-RET electron donor and acceptor, respectively. In this protocol, mesoporous carbon nanospheres were adopted to immobilize ECL reactant Ru(bpy)₃²⁺ and antibody via nafion to acquire the RET donor nanocomposites (MOCs/nafion/Ru(bpy)₃²⁺/antibody), which were tightly interconnected with epoxy group functionalized Fe₃O₄ nanoparticles. It is of vital importance that GNRs with exquisite rod shape were synthesized and exhibited a typical absorption peak at 650 nm to quench ECL signal of Ru(bpy)₃²⁺ effectively. In addition, the ECL emission decreased linearly with the logarithm of Aβ concentration in a wide linear range from 1.0 × 10⁻⁵ to 100 ng/mL with a detection limit of 4.2 × 10⁻⁶ ng/mL. Furthermore, distinctive and desirable properties were verified to declare the promise for being applicable to analyze the Aβ content in real Alzheimer's cerebrospinal fluid samples with satisfactory results.

电化学发光（ECL）分析已逐渐引起人们对生物医学分析的兴趣。本文提出了一种新的方法，通过构造夹心式ECL传感平台，可对阿尔茨海默氏病标记物β-淀粉样蛋白（Aβ）进行超灵敏的检测。在这里，电化学发光共振能量转移（ECL-RET）被用来确定Aβ浓度，这可以归因于Ru（bpy）₃ ²⁺与充当ECL-RET电子供体的金纳米棒（GNR）之间的RET的猝灭作用。受体。在该协议中，采用介孔碳纳米球通过nafion固定ECL反应物Ru（bpy）₃ ²⁺和抗体，以获得RET供体纳米复合材料（MOCs / nafion / Ru（bpy）₃ ²⁺/抗体）与环氧基官能化的Fe ₃ O ₄纳米颗粒紧密连接。至关重要的是，要合成出具有棒状形状的GNR，并在650 nm处表现出典型的吸收峰，以有效猝灭Ru（bpy）₃ ^2+的ECL信号。此外，ECL发射在1.0×10 ^-5到100 ng / mL的宽线性范围内随Aβ浓度的对数线性下降，检测极限为4.2×10 ^-6  ng / mL。此外，已验证了独特而理想的特性，以表明其有望用于分析实际阿尔茨海默氏病脑脊髓液样品中的Aβ含量，并获得令人满意的结果。