To date, existing tissue adhesives have various weak points in gluing kinetics and stability – particularly, in biocompatibility, which make most of them remain suboptimal for internal conditions. Herein, a novel mussel-inspired “BCD” tissue glue made of bovine serum albumin (BSA), citrate acid (CA) and dopamine was developed aiming at internal medical applications. BSA was employed as a natural and biocompatible macromolecular backbone; CA was introduced as a dual-functional intermediate to increase reactive carboxyl sites for engraftment of dopamine onto BSA backbone and also block the competing reactive amines from the proteinic backbone. Timely curing and stable adhesion were achieved between biological tissue substrates via instant chelation and gradual conjugation of DOPA-catechol groups in BCD glue. Within 30 min, this newly developed BCD tissue glue can provide over 10-fold greater adhesion stress than that of commercially available fibrin glue in wet environment. As a tissue adhesive for internal use, its superior properties also include ideal gelation kinetics and swelling behaviour, appropriate degradation rate, sound cytocompatibility in vitro, as well as fine biocompatibility in vivo. More importantly, successful animal experimentations in seroma prevention and instant hemostasis ultimately validated BCD tissue glue's preclinical efficacy as a tissue adhesive for various internal medical applications.

中文翻译：

---

一种新型的基于DOPA-白蛋白的组织粘合剂，可用于内部医疗应用

迄今为止，现有的组织粘合剂在胶合动力学和稳定性方面（尤其是在生物相容性方面）具有各种弱点，这使得它们中的大多数对于内部条件而言仍然不是最佳的。在本文中，针对内部医学应用，开发了一种由贻贝启发的，由牛血清白蛋白（BSA），柠檬酸（CA）和多巴胺制成的新型“ BCD”组织胶。BSA被用作天然和生物相容的高分子骨架。引入CA作为双功能中间体，以增加用于将多巴胺嫁接到BSA骨架上的反应性羧基位点，也可以阻止来自蛋白质骨架的竞争性反应性胺。通过BCD胶中的DOPA-儿茶酚基团的即时螯合和逐步结合，在生物组织基质之间实现了及时的固化和稳定的粘附。在30分钟内，这种新开发的BCD组织胶可以在潮湿环境中提供比市售纤维蛋白胶大10倍以上的粘合应力。作为内部使用的组织粘合剂，其优越的性能还包括理想的凝胶动力学和溶胀性能，适当的降解速率，体外良好的细胞相容性以及体内良好的生物相容性。更重要的是，在预防血清肿和即时止血方面成功的动物实验最终证实了BCD组织胶作为各种内部医疗应用的组织粘合剂的临床前功效。适当的降解速率，体外良好的细胞相容性以及体内良好的生物相容性。更重要的是，在预防血清肿和即时止血方面成功的动物实验最终证实了BCD组织胶作为各种内部医疗应用的组织粘合剂的临床前功效。适当的降解速率，体外良好的细胞相容性以及体内良好的生物相容性。更重要的是，在预防血清肿和即时止血方面成功的动物实验最终证实了BCD组织胶作为各种内部医疗应用的组织粘合剂的临床前功效。