This report outlines a rapid and highly sensitive colorimetric sensing platform based on a novel water-soluble WOx quantum dots (QDs) as enzymatic mimics. The WOx QDs, synthesized by a green hydrothermal method, were proven to possess excellent intrinsic peroxidase-like activity. The peroxidase substrate, 2, 2'-azinobis (3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt (ABTS) could be catalyzed oxidation by WOx QDs quickly in the present of H2O2 to form a green color. Due to its excellent properties such as excellent water-solublity, good biocompatibility, easy preparation, and high stability, the proposed system showed typical Michaelis-Menten kinetics and high affinity for ABTS and H2O2, indicating WOx QDs can be used as satisfactory peroxidase mimics. Together with the fast detection process, WOx QDs-based colorimetric sensing system provided a facile, sensitive, and accurate method for the determination of H2O2 and glucose. The real glucose samples in human serum samples were detected with satisfying results. The proposed sensing platform not only expands the applications of WOx and WOx QDs-based nanocomposites, but also provides an alternative technique for biological, biomedical and environmental sample assay.

中文翻译：

---

基于具有内在过氧化物酶活性的水溶性WO x 量子点的高灵敏度和快速比色传感平台

本报告概述了一种基于新型水溶性 WOx 量子点 (QD) 作为酶模拟物的快速且高度灵敏的比色传感平台。通过绿色水热法合成的 WOx QD 被证明具有优异的内在过氧化物酶样活性。过氧化物酶底物 2, 2'-azinobis (3-ethylbenzthiazoline-6-sulfonic acid) 二铵盐 (ABTS) 可以在 H2O2 存在下被 WOx QD 快速催化氧化，形成绿色。由于其优异的水溶性、良好的生物相容性、易于制备和高稳定性等优异特性，该系统表现出典型的 Michaelis-Menten 动力学和对 ABTS 和 H2O2 的高亲和力，表明 WOx QD 可用作令人满意的过氧化物酶模拟物。加上快速的检测过程，基于 WOx QDs 的比色传感系统为 H2O2 和葡萄糖的测定提供了一种简便、灵敏和准确的方法。对人血清样品中的真实葡萄糖样品进行检测，结果令人满意。所提出的传感平台不仅扩展了基于 WOx 和 WOx 量子点的纳米复合材料的应用，而且为生物、生物医学和环境样品分析提供了一种替代技术。