A method integrating capillary electrophoresis (CE) and gold nanoparticle aggregation-based colorimetry (AuNP-ABC) was described. By using a dual-sheath interface, the running buffer was isolated from the colorimetric reaction solution so that CE and AuNP-ABC would not interfere with each other. The proof-of-concept was validated by assay of polyamidoamine (PAMAM) dendrimers that were fortified in human urine samples. The factors influencing the CE-AuNP-ABC performances were investigated and optimized. Under the optimal conditions, the dendrimers were separated within 8 min, with detection limits of 0.5, 1.2 and 2.6 μg mL-1 for PAMAM G1.0, G2.0 and G3.0, respectively. The sensitivity of CE-AuNP-ABC was comparable to or even better than those of liquid chromatography-fluorimetry and liquid chromatography-mass spectrometry. The results suggested that the proposed strategy can be applied to facile and quick determination of analytes of similar properties in complex matrices.

中文翻译：

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毛细管电泳与基于金纳米颗粒的比色法的集成

描述了一种将毛细管电泳 (CE) 和基于金纳米颗粒聚集的比色法 (AuNP-ABC) 相结合的方法。通过使用双鞘接口，运行缓冲液与比色反应溶液隔离，因此 CE 和 AuNP-ABC 不会相互干扰。概念验证通过对人类尿液样本中强化的聚酰胺胺 (PAMAM) 树枝状聚合物的测定进行了验证。研究并优化了影响 CE-AuNP-ABC 性能的因素。在最佳条件下，树枝状大分子在 8 分钟内分离，PAMAM G1.0、G2.0 和 G3.0 的检出限分别为 0.5、1.2 和 2.6 μg mL-1。CE-AuNP-ABC的灵敏度与液相色谱-荧光法和液相色谱-质谱法相当甚至更好。