A NP-based biobarcode for C-reactive protein (CRP) quantification in plasma samples is reported for the first time. The assay uses capture antibody functionalized magnetic beads (pAbCRP2-MP), multifunctional oligonucleotide encoded probes modified with a detection antibody (pAbCRP1-ePSP), and a fluorescent DNA microarray. Thus, magnetic beads are added to the sample to form immunocomplexes that will be isolated, to then add the codified particles to form a sandwich complex with both particles and the target protein, subsequently the complexes are treated to release the oligonucleotide codes, which are finally hybridized in a fluorescent DNA microarray. The assay has been implemented to the analysis of plasma samples being able to quantify this biomarker within 900 ng mL-1 to 12500 ng mL-1 with an excellent accuracy (mean of recovery of 99.5 ± 4.2%, N = 3). The CRP biobarcode has been used on a small pilot clinical study in which plasma samples from patients suffering different pathologies, most of them related to cardiovascular diseases (CVDs). The samples have been analyzed and the results compared to a reference method demonstrating that the assay can be useful for monitoring this biomarker on patients being suspicious to be under risk of suffering CVDs or other diseases involving inflammatory processes.

中文翻译：

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基于三明治 NP 的生物条形码检测，用于定量血浆样品中的 C 反应蛋白

首次报道了用于血浆样品中 C 反应蛋白 (CRP) 定量的基于 NP 的生物条形码。该测定使用捕获抗体功能化磁珠 (pAbCRP2-MP)、用检测抗体 (pAbCRP1-ePSP) 修饰的多功能寡核苷酸编码探针和荧光 DNA 微阵列。因此，将磁珠加入样品中形成免疫复合物进行分离，然后加入编码颗粒与颗粒和目标蛋白形成夹心复合物，随后处理复合物以释放寡核苷酸密码，最终在荧光 DNA 微阵列中杂交。该测定已用于血浆样品的分析，能够在 900 ng mL-1 至 12500 ng mL-1 范围内以极好的准确度（平均回收率为 99.5 ± 4.2%，N = 3）。CRP 生物条形码已用于一项小型试点临床研究，其中来自患有不同病理的患者的血浆样本，其中大多数与心血管疾病 (CVD) 相关。对样品进行了分析，并将结果与​​参考方法进行了比较，表明该测定可用于监测疑似患有 CVD 或其他涉及炎症过程的疾病风险的患者的这种生物标志物。