A novel fluorescent and colorimetric sensor (WM-S) was synthesized from coumarin oxime and 2-chloroethyl methyl sulfide in a straightforward manner. The sensor displayed high selectivity for detection of Pd²⁺ over other metal ions (Cu²⁺ and Hg²⁺ in particular). The probe also exhibited high sensitivity for recognition of Pd²⁺ in aqueous media and can detect trace amounts of Pd²⁺ in real time, with a detection limit as low as 41.5 nM. Fluorescence imaging experiments of Pd²⁺ in HeLa cells indicated that the sensor can penetrate cell membranes and effectively detect intracellular Pd²⁺.

中文翻译：

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比色和荧光探针可实时检测水性介质中的钯（II）离子并进行活细胞成像

以香豆素肟和2-氯乙基甲基硫醚为原料，直接合成了一种新型的荧光比色传感器（WM-S）。该传感器显示出相对于其他金属离子（特别是Cu ²⁺和Hg ²⁺）检测Pd ^2+的高选择性。该探针还表现出对水性介质中Pd ²⁺识别的高灵敏度，并且可以实时检测痕量Pd ²⁺，检测限低至41.5 nM。HeLa细胞中Pd ^2+的荧光成像实验表明，该传感器可以穿透细胞膜并有效检测细胞内Pd ²⁺。