Detection of membrane-bound and soluble antigens by magnetic levitation,Lab on a Chip

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Detection of membrane-bound and soluble antigens by magnetic levitation
Lab on a Chip ( IF 6.1 ) Pub Date : 2017-09-05 00:00:00 , DOI: 10.1039/c7lc00402h
Mikkel Schou Andersen _{1,

1,

2,

3,

4} , Emily Howard _{2,

3,

4,

5,

6} , Shulin Lu _{2,

3,

4,

5,

6} , Matthew Richard _{2,

3,

4,

5,

6} , Mark Gregory _{2,

3,

4,

5,

6} , Gordon Ogembo _{2,

3,

4,

5,

6} , Ofer Mazor _{3,

4,

7} , Pavel Gorelik _{3,

4,

7} , Nathan I. Shapiro _{1,

2,

3,

4} , Anish V. Sharda _{2,

3,

4,

5,

6} , Ionita Ghiran _{2,

3,

4,

5,

6}

Affiliation

Magnetic levitation is a technique for measuring the density and the magnetic properties of objects suspended in a paramagnetic field. We describe a novel magnetic levitation-based method that can specifically detect cell membrane-bound and soluble antigens by measurable changes in levitation height that result from the formation of antibody-coated bead and antigen complex. We demonstrate our method's ability to sensitively detect an array of membrane-bound and soluble antigens found in blood, including T-cell antigen CD3, eosinophil antigen Siglec-8, red blood cell antigens CD35 and RhD, red blood cell-bound Epstein–Barr viral particles, and soluble IL-6, and validate the results by flow cytometry and immunofluorescence microscopy performed in parallel. Additionally, employing an inexpensive, single lens, manual focus, wifi-enabled camera, we extend the portability of our method for its potential use as a point-of-care diagnostic assay.

中文翻译：

磁悬浮法检测膜结合和可溶性抗原

磁悬浮是一种测量悬浮在顺磁场中的物体的密度和磁性的技术。我们描述了一种新型的基于磁悬浮的方法，该方法可以通过检测由抗体包被的磁珠和抗原复合物的形成而引起的悬浮高度的可测量变化来特异性检测细胞膜结合的可溶性抗原。我们证明了我们的方法能够灵敏地检测血液中发现的一系列膜结合和可溶性抗原，包括T细胞抗原CD3，嗜酸性粒细胞抗原Siglec-8，红细胞抗原CD35和RhD，红细胞结合的Epstein–Barr病毒颗粒和可溶性IL-6，并通过并行执行的流式细胞仪和免疫荧光显微镜验证结果。此外，使用廉价的单镜头，手动对焦，支持wifi的相机，

更新日期：2017-09-14

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