As a promising candidate of point-of-care devices (POC), digital microfluidic (DMF), driven by the electrowetting-on-dielectric (EWOD), has been developed quickly in recent years. However, flourishing functions require more controlled electrodes, i.e., a large-scale EWOD. Limited to cost, complexity and flexibility, existing control techniques lack strength in meeting the need of controlling increasing number of electrodes. Here, inspired by the active matrix electrowetting-on-dielectric (AM-EWOD), we present a “direct-address based on cross-control” (DACC) method, which can easily realize N + M pins controlling N × M electrodes and guarantee the control flexibility meanwhile. Moreover, combining with impedance detection technique, we set up a cheap and portable cyberphysical system. The system’s software, written in JAVA, has a user-friendly interface and can sense all sites of the DMF in real-time and auto-design routes for droplets to bypass barriers. This novel integrated digital microfluidic system shows great potential for developing low-cost, portable, and automated EWOD DMFs in large-scale.

作为即时护理设备（POC）的有前途的候选者，近年来，由介电体电润湿（EWOD）驱动的数字微流控（DMF）已得到快速发展。然而，繁华的功能需要更多可控的电极，即，大型EWOD。受成本，复杂性和灵活性的限制，现有的控制技术缺乏满足控制越来越多的电极的需求的强度。在这里，受有源矩阵电介质上电润湿（AM-EWOD）的启发，我们提出了一种“基于交叉控制的直接地址”（DACC）方法，该方法可以轻松实现控制N×M电极的N + M引脚和同时保证控制的灵活性。此外，结合阻抗检测技术，我们建立了一个便宜且便携式的网络物理系统。用JAVA编写的系统软件具有友好的用户界面，可以实时和自动设计路线感应DMF的所有位置，以使液滴绕过障碍。这种新颖的集成式数字微流控系统显示出开发低成本，便携式，