We report the development of a Fluorescence Resonance Energy Transfer (FRET) based sensor system for highly sensitive detection of nitro-aromatic compounds. The sensor system is composed of a copolymer (poly(methyl methacrylate (MMA))-co-7-(4-trifluoromethyl)coumarin-N-methacrylamide (TCMA)) (CP)¹ bearing coumarin derivative in the side chain as donor and curcumin (dye) as an acceptor. Primarily, the fluorescence of the above mentioned copolymer is quenched by non-radiative energy transfer to curcumin which occurs when the two molecules are within FRET distance scale. The efficiency of this FRET process was calculated. A noticeable decrease in the FRET signal is observed as nitroaromatics viz. 2,4-dinitrotoluene (DNT) and 2,4,6-trinitrotoluene (TNT) is gradually introduced into the sensor system. Fluorescence lifetime is measured to validate the efficacy of the energy transfer process in CP-curcumin sensor system. The mechanistic details of molecular interactions between CP and curcumin system are established by infrared spectroscopy and UV–vis absorption spectroscopy.

我们报告了基于荧光共振能量转移（FRET）的传感器系统的发展，用于高度敏感地检测硝基芳族化合物。传感器系统由共聚物（聚（甲基丙烯酸甲酯（MMA））- co -7-（4-三氟甲基）香豆素-N-甲基丙烯酰胺（TCMA））（CP）^1组成在侧链上带有香豆素衍生物作为供体，姜黄素（染料）作为受体。首先，上述共聚物的荧光通过非辐射能量转移至姜黄素而淬灭，这发生在两个分子在FRET距离范围内时。计算了该FRET过程的效率。随着硝基芳族化合物的观察，FRET信号明显降低。2,4-二硝基甲苯（DNT）和2,4,6-三硝基甲苯（TNT）逐渐引入传感器系统。测量荧光寿命以验证CP-姜黄素传感器系统中能量转移过程的功效。CP和姜黄素系统之间的分子相互作用的机械细节是通过红外光谱和UV-vis吸收光谱确定的。