A two-step fermentation strategy using glucose mother liquor (GML) for cell growth and xylose mother liquor (XML) for welan gum synthesis was used to alleviate uneconomic welan gum fermentation. This study revealed: (1) optimal initial GML concentration was 11.7 g/L (10 g/L sugars contained); (2) optimal XML feeding strategy was pseudo-exponential fed-batch and feeding time was 12th h to 54th h, amounting to 25.7 g/L XML (20 g/L sugars contained); and (3) in a 7.5-L bioreactor, welan gum concentration was 22.68 ± 0.50 g/L and its yield reached 0.756 g/g sugars with trace residual sugars. Compared with the cost of batch fermentation using glucose as sole carbon source, the final carbon source costs decreased by 61.40% and the welan gum yield increased by 50%. GML and XML can be used as inexpensive carbon sources for welan gum production with higher yield, giving them industrial application potential to produce value-added chemicals.

中文翻译：

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Sphingomonas sp。生产的两步经济的Welan胶。糖工业副产品中的HT-1

使用葡萄糖母液（GML）促进细胞生长和木糖母液（XML）促进Welan胶合成的两步发酵策略可缓解不经济的Welan胶发酵。该研究表明：（1）最佳初始GML浓度为11.7 g / L（含10 g / L糖）；（2）最佳的XML补料策略是伪指数补料，补料时间为12h至54h，总计XML量为25.7 g / L（含20 g / L糖）；（3）在7.5 L的生物反应器中，Welan胶的浓度为22.68±0.50 g / L，其产量达到0.756 g / g糖，并带有微量残留糖。与使用葡萄糖作为唯一碳源的分批发酵成本相比，最终碳源成本降低了61.40％，威兰胶产量提高了50％。