The inclusion complexes between 17-α-methyltestosterone (MT) and β-cyclodextrin (bCD) were prepared and characterized in dissolution and solid phase. The complex promoted a sixfold increment in solubility of the hormone. It has a limited solubility and stoichiometry of 2:1 (bCD:MT) determined by DSC, NMR and solubility experiments, the association constant Ka = 2846 L mol⁻¹ and complex fraction of 76% (assessed by DOSY-NMR, in (1:3) DMSO/D₂O). The association constant obtained in water by the solubility isotherms is 7540 L mol⁻¹. 2D-ROESY experiments indicate the intermolecular orientation (complete inclusion of the hormone in the cavity). Simulations by molecular dynamics agreed with the formation of the inclusion complex 2:1. Release tests showed the slower release for the complexes, with 50% for lyophilization and 56% for malaxation. These results clearly demonstrate the complexation of MT in bCD, which formulations are promising for further applications involving this steroid in aquaculture, both for sexual reversal and in technologies of hormone in water sequestration.

中文翻译：

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甲基睾丸激素和β-环糊精之间的配合物，用于水产养殖生产

制备了17-α-甲基睾丸酮（MT）和β-环糊精（bCD）之间的包合物，并通过溶解和固相表征。该复合物使激素的溶解度提高了六倍。通过DSC，NMR和溶解度实验确定其具有2：1（bCD：MT）的有限溶解度和化学计量，缔合常数Ka = 2846 L mol ^-1和76％的复杂分数（通过DOSY-NMR评估，在（ 1：3）DMSO / D ₂ O）。通过溶解度等温线在水中获得的缔合常数为7540 L mol ^-1。2D-ROESY实验表明分子间的方向（腔中激素的完全包含）。分子动力学模拟与包合物2：1的形成相吻合。释放测试表明复合物的释放较慢，冻干为50％，疟疾为56％。这些结果清楚地证明了MT在bCD中的复杂性，该制剂有望将这种类固醇用于水产养殖中，用于性逆转和水封固激素技术中。