A heteropolysaccharide was isolated by cold aqueous extraction from edible mushroom Pleurotus eryngii (“King Oyster”) basidiocarps and its biological properties were evaluated. Structural assignments were carried out using mono- and bidimensional NMR spectroscopy, monosaccharide composition, and methylation analyses. A mannogalactan having a main chain of (1 → 6)-linked α-d-galactopyranosyl and 3-O-methyl-α-d-galactopyranosyl residues, both partially substituted at OH-2 by β-d-Manp (MG-Pe) single-unit was found. Biological effects of mannogalactan from P. eryngii (MG-Pe) were tested against murine melanoma cells. MG-Pe was non-cytotoxic, but reduced in vitro melanoma cells invasion. Also, 50 mg/kg MG-Pe administration to melanoma-bearing C57BL/6 mice up to 10 days decreased in 60% the tumor volume compared to control. Additionally, no changes were observed when biochemical profile, complete blood cells count (CBC), organs, and body weight were analyzed. Mg-Pe was shown to be a promising anti-melanoma molecule capable of switching melanoma cells to a non-invasive phenotype with no toxicity to melanoma-bearing mice.

中文翻译：

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使用新型抗肿瘤药物，来自杏鲍菇的部分甲基化甘露半乳聚糖对小鼠黑色素瘤模型进行安全治疗

通过冷水提取从食用蘑菇杏鲍菇侧柏皮中分离出杂多糖，并对其生物学特性进行了评估。使用一维和二维NMR光谱，单糖组成和甲基化分析进行结构分配。具有（1→6）的主链的-连接的mannogalactanα- d -galactopyranosyl和3- ö甲基- α- d -galactopyranosyl残基，这两个部分在OH-2通过β-取代d -Man p（MG- Pe）单元被发现。从mannogalactan的生物学效应杏鲍菇（MG- Pe的）已针对鼠黑色素瘤细胞进行了测试。MG - Pe无细胞毒性，但减少了体外黑色素瘤细胞的侵袭。而且，与含黑素瘤的C57BL / 6小鼠相比，在长达10天的时间内给予50 mg / kg MG - Pe与对照组相比，其肿瘤体积降低了60％。此外，在分析生化特征，全血细胞计数（CBC），器官和体重时，未观察到任何变化。Mg- Pe被证明是一种有前途的抗黑素瘤分子，能够将黑素瘤细胞切换为非侵入性表型，而对荷黑素瘤的小鼠没有毒性。