Rosmarinic acid-4-O-β-D-glucoside (RAG) is a dicaffeoyl phenolic compound isolated from Sarcandra glabra (Thunb.) Nakai. Preliminary studies show that RAG has significant anti-inflammatory properties and can alleviate ear swelling in mice and the paw swelling in rats. Here, the anti-influenza effects of RAG were investigated in mice infected with A/FM/1/47 H1N1 virus. The survival rate and body weight were observed, the lung edema, virus copies, inflammatory cytokines (including IL-4, IL-5, TNF-α and IFN-γ) and oxidative damage indexes (including SOD, MDA, NO, and CAT) were measured. Moreover, immune cell recruitment in alveoli was measured with white blood cells and differential counts. Therapeutic RAG concentrations substantially improve the symptoms, mitigate body weight loss and alleviate lung edema induced by virus, thus improve survival protection effects. Furthermore, RAG was shown to regulate influenza virus-induced inflammatory cytokine expression, specifically by downregulating the Th1 cell cytokines IFN-γ, TNF-α and upregulating the Th2 cell cytokines IL-4, IL-5. Cell migration and infiltration were also diminished after RAG administration.

Rosmarinic Acid-4-O-β-D-glucoside (RAG) 是一种从草珊瑚(Thunb.) Nakai 中分离出来的二咖啡酰酚类化合物。初步研究表明，RAG 具有显着的抗炎特性，可以缓解小鼠耳肿胀和大鼠爪肿胀。在此，我们在感染 A/FM/1/47 H1N1 病毒的小鼠中研究了 RAG 的抗流感作用。观察存活率、体重、肺水肿、病毒拷贝数、炎性细胞因子（包括IL-4、IL-5、TNF-α、IFN-γ）和氧化损伤指标（包括SOD、MDA、NO、CAT） ）进行了测量。此外，通过白细胞和分类计数来测量肺泡中免疫细胞的募集。治疗性RAG浓度可显着改善症状，减轻体重减轻，减轻病毒引起的肺水肿，从而提高生存保护效果。此外，RAG 还可以调节流感病毒诱导的炎症细胞因子表达，特别是通过下调 Th1 细胞细胞因子 IFN-γ、TNF-α 和上调 Th2 细胞细胞因子 IL-4、IL-5。 RAG 施用后细胞迁移和浸润也减少。