The present study examines the susceptibility of mouse induced pluripotent stem cell-derived neural precursor cells (iPSC-NPCs) to infection with the neurotropic JHM strain of mouse hepatitis virus (JHMV). Similar to NPCs derived from striatum of day 1 postnatal GFP-transgenic mice (GFP-NPCs), iPSC-derived NPCs (iPSC-NPCs) are able to differentiate into terminal neural cell types and express MHC class I and II in response to IFN-γ treatment. However, in contrast to postnatally-derived NPCs, iPSC-NPCs express low levels of carcinoembryonic antigen-cell adhesion molecule 1a (CEACAM1a), the surface receptor for JHMV, and are less susceptible to infection and virus-induced cytopathic effects. The relevance of this in terms of therapeutic application of NPCs resistant to viral infection is discussed.

本研究检查了小鼠诱导的多能干细胞源性神经前体细胞（iPSC-NPC）对小鼠肝炎病毒（JHMV）的神经性JHM株感染的敏感性。与从出生后第1天的GFP转基因小鼠的纹状体衍生的NPC（GFP-NPC）相似，iPSC衍生的NPC（iPSC-NPC）能够分化为终末神经细胞类型，并响应IFN-γ表达MHC I类和II类。 γ处理。但是，与出生后的NPC相比，iPSC-NPC表达低水平的JHMV表面受体癌胚抗原-细胞粘附分子1a（CEACAM1a），并且不易受感染和病毒诱导的细胞病变作用。讨论了在抗病毒感染的NPC的治疗应用方面的相关性。