The RNA polymerase complex (RNApc) in influenza A viruses (IVs) is composed of the PB2, PB1 and PA subunits, which are encoded by the three longest genome segments (Seg1-3) and are responsible for the replication of vRNAs and transcription of viral mRNAs. However, the co-evolutionary relationships of the three segments from the known 126 subtypes IVs are unclear. In this study, we performed a detailed analysis based on a total number of 121,191 nucleotide sequences. Three segment sequences were aligned before the repeated, incomplete and mixed sequences were removed for homologous and phylogenetic analyses. Subsequently, the estimated substitution rates and TMRCAs (Times for Most Recent Common Ancestor) were calculated by 175 representative IVs. Tracing the cladistic distribution of three segments from these IVs, co-evolutionary patterns and trajectories could be inferred. The further correlation analysis of six internal protein coding segments reflect the RNApc segments have the closer correlation than others during continuous reassortments. This global approach facilitates the establishment of a fast antiviral strategy and monitoring of viral variation.

甲型流感病毒（IV）中的RNA聚合酶复合物（RNApc）由PB2，PB1和PA亚基组成，它们由三个最长的基因组片段（Seg1-3）编码，并负责vRNA的复制和转录。病毒mRNA。但是，来自已知的126个亚型IV的三个片段的共同进化关系尚不清楚。在这项研究中，我们基于总数为121,191个核苷酸序列进行了详细的分析。在去除重复的，不完整的和混合的序列以进行同源和系统发育分析之前，对三个片段序列进行比对。随后，通过175个代表性IV来计算估计的替代率和TMRCA（最近的共同祖先的时间）。追踪这些IV的三个部分的分类分布，可以推断出共同进化的模式和轨迹。六个内部蛋白质编码片段的进一步相关性分析表明，在连续重排期间，RNApc片段的相关性高于其他片段。这种全球方法有助于建立快速的抗病毒策略和监测病毒变异。