The proteome and phosphoproteome of non-structural proteins of Adenovirus type 2 (Ad2) were time resolved using a developed mass spectrometry approach. These proteins are expressed by the viral genome and important for the infection process, but not part of the virus particle. We unambiguously confirm the existence of 95% of the viral proteins predicted to be encoded by the viral genome. Most non-structural proteins peaked in expression at late time post infection. We identified 27 non-redundant sites of phosphorylation on seven different non-structural proteins. The most heavily phosphorylated protein was the DNA binding protein (DBP) with 15 different sites. The phosphorylation occupancy rate could be calculated and monitored with time post infection for 15 phosphorylated sites on various proteins. In the DBP, phosphorylations with time-dependent relation were observed. The findings show the complexity of the Ad2 non-structural proteins and opens up a discussion for potential new drug targets.

使用发达的质谱方法对2型腺病毒（Ad2）非结构蛋白的蛋白质组和磷酸化蛋白质组进行时间分辨。这些蛋白质由病毒基因组表达，对感染过程很重要，但不是病毒颗粒的一部分。我们明确确认了95％的病毒蛋白预计会被病毒基因组编码。大多数非结构蛋白在感染后的晚期达到表达高峰。我们在七个不同的非结构蛋白上鉴定了27个磷酸化的非冗余位点。磷酸化程度最高的蛋白是具有15个不同位点的DNA结合蛋白（DBP）。感染后的时间可以计算并监测各种蛋白质上15个磷酸化位点的磷酸化占有率。在DBP中，观察到具有时间依赖性关系的磷酸化。这些发现表明了Ad2非结构蛋白的复杂性，并为潜在的新药靶标展开了讨论。