A new hydroponic study design to determine uptake of chemicals by plant roots was tested by (i) investigating uptake of [¹⁴C]-1,2,4-triazole by wheat plants in a ring test with ten laboratory organizations and (ii) studying uptake of ten other radiolabelled chemicals by potato, tomato or wheat plants in two laboratories. Replicate data from the ring test were used to calculate plant uptake factor (PUF) values (uptake into roots and shoots) and transpiration stream concentration factor (TSCF) values (uptake into shoots). Average PUF for 1,2,4-triazole was 0.73 (n = 39, 95% confidence interval (CI): 0.64, 0.82) and the corresponding TSCF value was 1.03 (n = 49, 95% CI: 0.76, 1.3). Boxplots and subsequent classification tree analysis of PUF and TSCF values showed that potential outlier values were > 1.38 and were observed for PUF replicates with low biomass increase (ratio of final to initial biomass ≤ 1.739) and small initial biomass (≤ 1.55 g) and for TSCF replicates with an increase in biomass of < 0.67 g over a period of eight days. Considering only valid replicate data, average values of PUF and TSCF were 0.65 (n = 33, 95% CI: 0.57, 0.73) and 0.64 (n = 39, 95% CI: 0.58, 0.70). The additional experiments with ten chemicals and three plant species showed that uptake was low for polar substances of high molecular weight (≥ 394 g/mol) and that TSCF values increased with log K_ow values of the tested chemicals ranging from − 1.54 to 1.88 (polynomial equation with R² = 0.64). A cluster analysis for three of the compounds that were tested on wheat and tomato indicated that the plant uptake was mainly determined by the substance. Overall, the findings show that the hydroponic study design allows for reliable quantification of plant uptake over a range of compound/crop combinations.

中文翻译：

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评估新型测试设计以确定植物根系对化学物质的吸收

通过（i）与十个实验室组织进行的环试验研究小麦植物对[ ¹⁴ C] -1,2,4-三唑的吸收，从而确定植物根系对化学物质吸收的新水培研究设计。在两个实验室中，马铃薯，番茄或小麦植物吸收了十种其他放射性标记的化学物质。环试验的重复数据用于计算植物吸收因子（PUF）值（吸收到根和芽中）和蒸腾流集中因子（TSCF）值（吸收到芽中）。1,2,4-三唑的平均PUF为0.73（n  = 39，95％置信区间（CI）：0.64，0.82），相应的TSCF值为1.03（n = 49，95％CI：0.76，1.3）。箱形图和随后的PUF和TSCF值分类树分析显示，潜在离群值大于1.38，并且在生物量增加低（最终生物量与初始生物量之比≤1.739）和较小初始生物量（≤1.55 g）的PUF复制品中观察到。 TSCF在八天的时间内生物量增加了<0.67 g，可进行复制。仅考虑有效的重复数据，PUF和TSCF的平均值分别为0.65（n  = 33，95 ％CI：0.57，0.73）和0.64（n  = 39，95％CI：0.58，0.70）。用十种化学物质和三种植物物种进行的附加实验表明，高分子量（≥394 g / mol）的极性物质的摄取较低，并且TSCF值随log K _ow的增加而增加被测化学物质的值在− 1.54至1.88之间（R ²  = 0.64的多项式方程）。对在小麦和番茄上测试的三种化合物进行的聚类分析表明，植物吸收主要由该物质决定。总体而言，研究结果表明，水培研究设计可对一系列化合物/作物组合中的植物吸收进行可靠的量化。