Bam35 is the paradigm of the membrane-containing tectivirus group, which infect hosts from the Bacillus cereus sensu lato group. Due to their ancient origin and complex diversity, many tectiviral proteins are ORFans of unknown function. Using yeast-two hybrid analyses, Berjón-Otero et al. (e00870-17) defined a comprehensive intraviral interactome that reveals functions for many viral proteins and suggests biological roles and locations within the viral particle of viral ORFan proteins. These findings lead to many hypotheses for future work on tectivirus biology, diversity, and evolution.

Intraviral protein-protein interaction map of bacteriophage Bam35 proteins.

Bam35是含膜的tectivirus组的范例，该病毒感染蜡样芽胞杆菌组的宿主。由于其古老的起源和复杂的多样性，许多技术病毒蛋白是功能未知的ORFans。Berjón-Otero等人使用酵母-两种杂交分析方法。（e00870-17）定义了一个全面的病毒内相互作用组，该相互作用组揭示了许多病毒蛋白的功能，并暗示了病毒ORFan蛋白在病毒颗粒内的生物学作用和位置。这些发现导致了关于假病毒生物学，多样性和进化的未来工作的许多假设。

噬菌体Bam35蛋白的病毒内蛋白-蛋白相互作用图。