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Highly Sensitive Dopamine Detection Using a Bespoke Functionalised Carbon Nanotube Microelectrode array
Electroanalysis ( IF 2.7 ) Pub Date : 2017-07-19 , DOI: 10.1002/elan.201700248
James Clark 1 , Ying Chen 2, 3 , Steven Hinder 4 , S. Ravi P. Silva 1
Affiliation  

Understanding how the brain works requires developing advanced tools that allow measurement of bioelectrical and biochemical signals, including how they propagate between neurons. The introduction of nanomaterials as electrode materials has improved the impedance and sensitivity of microelectrode arrays (MEAs), allowing high quality recordings of single cells in situ using electrode diameters of ≤20 μm. MEAs also have the potential to measure electroactive biological molecules in situ, such as dopamine, a neurotransmitter in the nervous system. Thus, this work focused on fabricating a functionalised carbon nanotube (CNT)-based MEA to demonstrate its potential for future measurement of small signals generated from excitable cells. To this end, the functionalised CNT MEA has recorded one of the lowest electrochemical interfacial impedances available in the literature, 2.8±0.2 kΩ, for an electrode of its geometric surface area. Electrochemical detection of dopamine revealed again one of the best sensitivity values per area available in the literature, 9.48 μA μM−1 mm−2. Additionally, a limit of detection of 7 nM was recorded for dopamine using the functionalised CNT MEA, with selectivity against common electrochemical interferents such as ascorbic acid. These results indicate improvement beyond currently available MEAs, along with the feasibility of using these devices for multi-site detection of physiologically relevant electroactive biomolecules.

中文翻译:

使用定制的功能化碳纳米管微电极阵列进行高灵敏度多巴胺检测

了解大脑如何工作需要开发先进的工具来测量生物电和生化信号,包括它们如何在神经元之间传播。纳米材料作为电极材料的引入提高了微电极阵列 (MEA) 的阻抗和灵敏度,允许使用≤20 μm 的电极直径对单个细胞进行高质量的原位记录。MEA 还具有在原位测量电活性生物分子的潜力,例如多巴胺,一种神经系统中的神经递质。因此,这项工作的重点是制造基于功能化碳纳米管 (CNT) 的 MEA,以证明其在未来测量可兴奋细胞产生的小信号方面的潜力。为此,功能化的 CNT MEA 记录了文献中可用的最低电化学界面阻抗之一,2.8±0.2 kΩ,用于其几何表面积的电极。多巴胺的电化学检测再次揭示了文献中可用的每个区域的最佳灵敏度值之一,9.48 μA μM-1 mm-2。此外,使用功能化的 CNT MEA 记录了多巴胺的检测限为 7 nM,对常见的电化学干扰物(如抗坏血酸)具有选择性。这些结果表明,除了目前可用的 MEA 之外,还有使用这些设备进行生理相关电活性生物分子多位点检测的可行性。多巴胺的电化学检测再次揭示了文献中可用的每个区域的最佳灵敏度值之一,9.48 μA μM-1 mm-2。此外,使用功能化的 CNT MEA 记录了多巴胺的检测限为 7 nM,对常见的电化学干扰物(如抗坏血酸)具有选择性。这些结果表明,除了目前可用的 MEA 之外,还有使用这些设备进行生理相关电活性生物分子多位点检测的可行性。多巴胺的电化学检测再次揭示了文献中可用的每个区域的最佳灵敏度值之一,9.48 μA μM-1 mm-2。此外,使用功能化的 CNT MEA 记录了多巴胺的检测限为 7 nM,对常见的电化学干扰物(如抗坏血酸)具有选择性。这些结果表明,除了目前可用的 MEA 之外,还有使用这些设备进行生理相关电活性生物分子多位点检测的可行性。
更新日期:2017-07-19
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