Novel Enzymatic Rhodium Modified Poly(styrene‐g‐oleic amide) Film Electrode for Hydrogen Peroxide Detection,Electroanalysis

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Novel Enzymatic Rhodium Modified Poly(styrene‐g‐oleic amide) Film Electrode for Hydrogen Peroxide Detection
Electroanalysis ( IF 2.7 ) Pub Date : 2017-07-19 , DOI: 10.1002/elan.201700332
Muhammet Samet Kilic ₁ , Seyda Korkut ₂ , Baki Hazer ₁

Affiliation

Newly synthesized poly(styrene‐g‐oleic amide) was coated onto a rhodium nanoparticle modified glassy carbon (GC) surface for the fabrication of horseradish peroxidase based biosensor used for hydrogen peroxide detection. The rhodium modifed electrode presented ten times higher signal than unmodified electrode even at low elecrtroactive enzyme quantity by enhancing the electron transfer rate at the applied potential of −0.65 V. The biosensor designed by under the optimized rhodium electrodeposition time exhibited a fast response less than 5 s, an excellent operational stability with a relative standard deviation of 0.6 % (n=6), an accuracy of 96 % and a large linear range between 50 μM and 120 mM for hydrogen peroxide. Detection limit and the sensitivity parameters were calculated to be 44 μM and 57 μA mM⁻¹ cm⁻², respectively by preserving its entire initial response up to the 15 days, while only 20 % of its initial response was lost at the end of one month.

中文翻译：

新型酶催化铑修饰的聚苯乙烯-g-油酰胺膜电极的过氧化氢检测

将新合成的聚（苯乙烯-g-油酰胺）涂在铑纳米粒子修饰的玻碳（GC）表面上，用于制造基于辣根过氧化物酶的生物传感器，用于过氧化氢检测。通过在-0.65 V的施加电势下提高电子传输速率，即使在低电活性酶的情况下，铑修饰的电极仍比未修饰的电极高十倍的信号。在优化的铑电沉积时间下设计的生物传感器显示出小于5的快速响应s，出色的操作稳定性，相对标准偏差为0.6％（n = 6），准确度为96％，过氧化氢的线性范围在50μM至120 mM之间。检测限和灵敏度参数经计算为44μM和57μAmM^-1 cm ^-2，通过保留其整个初始响应直到15天，而在一个月末仅损失了20％的初始响应。

更新日期：2017-07-19

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