Electrochemical Determination of Natural Drug Colchicine in Pharmaceuticals and Human Serum Sample and its Interaction with DNA,Electroanalysis

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Electrochemical Determination of Natural Drug Colchicine in Pharmaceuticals and Human Serum Sample and its Interaction with DNA
Electroanalysis ( IF 2.7 ) Pub Date : 2017-07-12 , DOI: 10.1002/elan.201700233
Dalibor M. Stanković _{1,

2} , Ľubomir Švorc ₃ , José F. M. L. Mariano ₄ , Astrid Ortner ₅ , Kurt Kalcher ₅

Affiliation

Colchicine (COLC) is a natural toxic product and secondary metabolite most commonly used to treat gout. In this study, its electrochemical behavior and determination was investigated by employing modification-free boron-doped diamond electrode (BDDE). Besides, its interaction with DNA was monitored using electrochemical methods. It was found that oxidation of this compound proceeds in two steps, where first sharp and well defined oxidation peak occurs at potential of around 1.19 V, and second one at around 1.37 V, in Britton-Robinson buffer solution at pH 7.5. Wide dynamic range from 1 to 100 μM was obtained with a detection limit (3σintercept/slope) of a 0.26 μM, based on the evaluation of first oxidation peak using differential pulse voltammetry. The proposed method was also found to be suitable for monitoring interaction of this drug with DNA as important segment for medical use. Concerning the validation, the analytical procedure shows excellent selectivity and sensitivity toward COLC detection and after method development it was successfully used for its quantification in pharmaceutical preparation and human serum sample, with satisfactory recovery. Obviously, this approach can be promising replacement for time-consuming and expensive separation methods.

中文翻译：

药物和人血清样品中天然药物秋水仙碱的电化学测定及其与DNA的相互作用

秋水仙碱 (COLC) 是一种天然有毒产品和次生代谢物，最常用于治疗痛风。在本研究中，采用无修饰的掺硼金刚石电极（BDDE）研究了其电化学行为和测定。此外，使用电化学方法监测其与 DNA 的相互作用。发现该化合物的氧化分两步进行，在 pH 7.5 的 Britton-Robinson 缓冲溶液中，第一个尖锐且明确的氧化峰出现在约 1.19 V 的电位处，第二个出现在约 1.37 V 处。基于使用差分脉冲伏安法对第一个氧化峰的评估，获得了从 1 到 100 μM 的宽动态范围，检测限（3σ截距/斜率）为 0.26 μM。还发现所提出的方法适用于监测该药物与作为医疗用途重要片段的 DNA 的相互作用。在验证方面，该分析程序对 COLC 检测显示出出色的选择性和灵敏度，在方法开发后，它成功用于药物制剂和人血清样品的定量，回收率令人满意。显然，这种方法有望替代耗时且昂贵的分离方法。

更新日期：2017-07-12

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