Simple and Fast Picomolar Detection of Ochratoxin A Using a Reusable Label Free Aptasensor Built with a Layer-by-layer Procedure,Electroanalysis

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Simple and Fast Picomolar Detection of Ochratoxin A Using a Reusable Label Free Aptasensor Built with a Layer-by-layer Procedure
Electroanalysis ( IF 2.7 ) Pub Date : 2017-07-11 , DOI: 10.1002/elan.201700290
Estéfani P. Simão ₁ , Roberto Cao-Milán ₂ , Graciela Costa-Pedro ₃ , Celso P. De Melo ₄ , Roberto Cao ₂ , Maria D. L. Oliveira ₁ , Cesar A. S. Andrade ₁

Affiliation

A label free impedimetric aptasensor for simple, fast and reusable picomolar detections of Ochratoxin A (OTA) in grape juices was designed. Two main factors were observed to affect the accurate detections of the toxin: i-lateral interactions between self-assembled aptamers ii- adsorption of large molecules present in complex matrixes like grape juices. Lateral interactions between aptamers were minimized by constructing the aptasensor in a Layer-by-Layer procedure. The interferences associated to the unspecific and irreversible adsorption of large molecules present in grape juice, were reduced by submitting samples to ultrafiltration prior to analysis. With this protocol, a 0.12 pM limit of detection and 0.24 pM limit of quantification in spiked grape juices were achieved after only 5–7 mins of interaction with the samples. The presented aptasensor can be recovered after a simple immersion in hot water (90 °C) for ten minutes.

中文翻译：

使用逐层程序构建的可重复使用的无标签适体传感器简单快速地检测赭曲霉毒素 A

设计了一种无标记阻抗适体传感器，用于简单、快速和可重复使用的葡萄汁中赭曲霉毒素 A (OTA) 的皮摩尔检测。观察到影响毒素准确检测的两个主要因素：自组装适体之间的 i-横向相互作用 ii-存在于复杂基质（如葡萄汁）中的大分子的吸附。通过在逐层程序中构建适体传感器，使适体之间的横向相互作用最小化。通过在分析前将样品进行超滤，可以减少与葡萄汁中存在的大分子的非特异性和不可逆吸附相关的干扰。使用该方案，在与样品相互作用仅 5-7 分钟后，加标葡萄汁的检测限为 0.12 pM，定量限为 0.24 pM。

更新日期：2017-07-11

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