Multiple displacement amplification (MDA), a most popular isothermal whole genome amplification (WGA) method, suffers the major hurdle of highly uneven amplification, thus, leading to many problems in approaching biological applications related to copy-number assessment. In addition to the optimization of reagents and conditions, complete physical separation of the entire reaction system into numerous tiny chambers or droplets using microfluidic devices, has been proven efficient to mitigate this amplifying bias in recent works. Here, we present another MDA advance, microchannel MDA (μcMDA), which decentralizes MDA reagents throughout a one-dimensional slender tube. Due to the double effect from soft partition of high molecular-weight DNA molecules and less-limited diffusion of small particles, μcMDA is shown to be significantly effective at improving the amplification uniformity, which enables us to accurately detect single nucleotide variants (SNVs) with higher efficiency and sensitivity. More importantly, this straightforward method requires neither customized instruments nor complicated operations, making it a ready-to-use technique in almost all biological laboratories.

中文翻译：

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一维反应器分散式MDA，可实现均匀，准确的全基因组扩增

多重置换扩增（MDA）是一种最流行的等温全基因组扩增（WGA）方法，存在着高度不均匀扩增的主要障碍，因此，在处理与拷贝数评估有关的生物学应用时会出现许多问题。除了优化试剂和条件外，最近的工作还证明了使用微流控设备将整个反应系统完全物理分离成许多小腔室或液滴的方法，可以有效缓解这种放大偏差。在这里，我们介绍了另一种MDA进展，即微通道MDA（μcMDA），它使一维细长管中的MDA试剂分散。由于高分子量DNA分子的软分配和小颗粒的扩散受限制的双重影响，已显示μcMDA在改善扩增均匀性方面非常有效，这使我们能够以更高的效率和灵敏度准确检测单核苷酸变体（SNV）。更重要的是，这种简单的方法不需要定制的仪器，也不需要复杂的操作，因此几乎在所有生物实验室中都可以立即使用。