Quantitative correction of matrix effects in the determination of copper in human serum by isotope dilution quadrupole ICP-MS,Journal of Analytical Atomic Spectrometry

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Quantitative correction of matrix effects in the determination of copper in human serum by isotope dilution quadrupole ICP-MS
Journal of Analytical Atomic Spectrometry ( IF 3.1 ) Pub Date : 2017-08-18 00:00:00 , DOI: 10.1039/c7ja00214a
Liuxing Feng _{1,

2,

3,

4} , Jun Wang _{1,

2,

3,

4} , Hongmei Li _{1,

2,

3,

4} , Yanjie Cui _{1,

2,

3,

4} , Hai Lu _{1,

2,

3,

4} , Naijie Shi _{1,

2,

3,

4}

Affiliation

Isotope Dilution Inductively Coupled Plasma Mass Spectrometry (ID-ICP-MS) has been applied to the accurate determination of elements in human serum. However, in the determination of Cu, because of high Na concentration (approximate 3500 μg g⁻¹) and other potential interfering elements (such as Ca, Mg, P and S), it is often a considerably difficult matter to accurately determine the ⁶³Cu/⁶⁵Cu ratio in the isotope dilution quadrupole ICP-MS (ID-Q-ICP-MS) procedure. In this study, a method for the quantitative correction of matrix effects is presented in the analysis of Cu in a human serum sample by ID-Q-ICP-MS. The potential matrix effects induced by non-spectral and spectral interference are fully investigated, and the polyatomic interference caused by high concentration of Na is proved to be the most important source of matrix effects in Cu determination. On this basis, a series of simulated Na matrix solutions with different ⁶³Cu/⁶⁵Cu ratios (from 0.1158 to 2.2440) are prepared to confirm the matrix correction factor, which is used for matrix correction after subsequent standard-sample bracketing (SSB) mass bias correction during ID-ICP-MS analysis. Moreover, this methodology is validated by using high resolution (HR) ICP-MS, and the consistent results prove the validity of this quantitative correction strategy. This idea will help to open the way to certain applications involving isotope ratio measurements, which previously have been beyond the reach of quadrupole ICP-MS instruments.

中文翻译：

同位素稀释四极杆ICP-MS测定人血清中的铜时基质效应的定量校正

同位素稀释电感耦合等离子体质谱法（ID-ICP-MS）已用于准确测定人血清中的元素。但是，在测定Cu时，由于Na浓度高（约3500μgg ^-1）和其他潜在的干扰元素（例如Ca，Mg，P和S），准确地确定⁶³铜/ ⁶⁵同位素稀释四极杆ICP-MS（ID-Q-ICP-MS）程序中的铜比。在这项研究中，通过ID-Q-ICP-MS分析人体血清样品中的Cu，提出了一种定量校正基质效应的方法。充分研究了非光谱和光谱干扰引起的潜在基体效应，事实证明，高浓度的Na引起的多原子干扰是Cu测定中基体效应的最重要来源。在此基础上，一系列具有不同⁶³ Cu / ^65的Na矩阵模拟溶液制备铜比（0.1158至2.2440）以确认基质校正因子，该因子用于在ID-ICP-MS分析过程中随后进行的标准样品包围（SSB）质量偏差校正后用于基质校正。此外，该方法已通过使用高分辨率（HR）ICP-MS进行了验证，并且一致的结果证明了这种定量校正策略的有效性。这个想法将有助于为某些涉及同位素比测量的应用开辟道路，而以前，这是四极杆ICP-MS仪器无法实现的。

更新日期：2017-09-13

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