当前位置: X-MOL 学术Adv. Funct. Mater. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Fluorescent Protein Nanovessels: A New Platform to Generate Bio–Abiotic Hybrid Materials for Bioimaging
Advanced Functional Materials ( IF 18.5 ) Pub Date : 2017-09-13 , DOI: 10.1002/adfm.201702051
Xiaoliang Wang 1 , Jianxu Zhang 2 , Youjun Wang 3 , Yang Liu 3 , Zhenhua Li 1 , Zhigang Xie 2 , Zhijun Chen 1
Affiliation  

In this report, a new platform to generate fluorescent protein nanovessels is described. Based on systemic analyses and reconstitution experiments, a combination of protein scaffold and organic dye is identified. Briefly, certain proteins such as bovine serum albumin (BSA) can rapidly form cube‐like scaffold upon heating. This protein scaffolds intrinsically interact with nonfluorescent dyes such as bromophenol blue (BPB), forming BSA‐BPB nanocubes (BBNCs). Moreover, it turns out that the commercially available dye BPB contains aggregation‐induced emission (AIE) properties, allowing the BBNCs emissive upon irradiation. The fluorescent protein nanovessels are highly biocompatible and can be readily internalized by different type of cells. The fluorescent signal of the materials is well‐penetrable from mouse tissues and can be detected at near‐infrared region, making it a useful tool for various biological imaging studies. This platform for making fluorescent protein nanovessels is green, rapid, and cost‐effective and can be extended to other protein scaffolds and possibly other dye/AIE molecules.

中文翻译:

荧光蛋白纳米血管:产生用于生物成像的生物非生物杂化材料的新平台

在此报告中,描述了产生荧光蛋白纳米血管的新平台。基于系统分析和重建实验,确定了蛋白质支架和有机染料的组合。简而言之,某些蛋白质(例如牛血清白蛋白(BSA))在加热时会迅速形成立方体状的支架。这种蛋白质支架与非荧光染料如溴酚蓝(BPB)本质上相互作用,形成BSA-BPB纳米立方体(BBNC)。而且,事实证明,市售染料BPB具有聚集诱导发射(AIE)特性,允许BBNC在照射时发光。荧光蛋白纳米血管具有高度的生物相容性,可以很容易地被不同类型的细胞内化。该材料的荧光信号可以很好地穿透小鼠组织,并且可以在近红外区域检测到,从而使其成为各种生物学成像研究的有用工具。这种制造荧光蛋白纳米血管的平台是绿色的,快速的,具有成本效益的,并且可以扩展到其他蛋白支架以及可能的其他染料/ AIE分子。
更新日期:2017-09-13
down
wechat
bug