A simple and efficient approach for the preparation of a biosensing platform was developed based on newly designed peptide-SNS type monomer conjugates. The approach involves the electrochemical polymerization of the peptide-SNS type monomer on the electrode surface. To synthesize the peptide bearing monomers, the SNS-type monomer having a carboxylic acid functional group was anchored to the C-terminal of the peptide by solid phase peptide synthesis via coupling reagents. Utilization of peptides to increase the solubility of the monomers was first investigated in this report. The obtained monomers, soluble in water, were fully characterized by spectral analyses and utilized as matrices for biomolecule attachment. Polymerization of monomers in water has the potential to provide an alternative process for the electrochemical preparation of the polymers in aqueous media, without using any organic solvent. Under the optimized conditions, the biosensor responded to the target analyte, glucose, in a strikingly selective and sensitive manner, and showed promising feasibility for the quantitative analysis of glucose in beverages.

中文翻译：

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水溶性肽-SNS型单体共轭物的聚合作用及其在生物传感器中的应用

基于新设计的肽-SNS型单体共轭物，开发了一种简单高效的生物传感平台制备方法。该方法涉及肽-SNS型单体在电极表面上的电化学聚合。为了合成带有肽的单体，通过固相肽合成，通过羧酸固溶，将具有羧酸官能团的SNS型单体锚定在肽的C-末端。偶联剂。在本报告中首先研究了利用肽增加单体的溶解度。通过光谱分析充分表征了所得的可溶于水的单体，并将其用作生物分子附着的基质。单体在水中的聚合有潜力提供一种在不使用任何有机溶剂的情况下在水性介质中电化学制备聚合物的替代方法。在最佳条件下，该生物传感器以惊人的选择性和灵敏方式对目标分析物葡萄糖作出反应，并显示了用于饮料中葡萄糖定量分析的有希望的可行性。