Herein, dual-functionalized reduced graphene oxide (rGO) hybrids (rGO/AuNP/luminol) with gold nanoparticles (AuNPs) and luminol were prepared via π–π conjugation and electrostatic interaction. The as-prepared rGO/AuNP/luminol hybrids exhibited enhanced and long-persistent chemiluminescence (CL) performance without decay for one hour when mixed with H₂O₂; this was due to the generation of ˙OH, O₂˙⁻, ¹O₂, and π–CC˙ species. A sensitive and selective CL sensor based on the rGO/AuNP/luminol hybrid CL enhancing system was developed for cholesterol quantitation in the linear range from 0.71 to 11.43 μM with a detection limit of 0.55 μM. Furthermore, the proposed method was successfully exploited for the determination of cholesterol in human serum samples.

中文翻译：

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鲁米诺和金纳米颗粒共沉淀的还原型氧化石墨烯杂化物，具有持久的化学发光能力，可用于胆固醇检测

本文中，通过π-π共轭和静电相互作用制备了具有金纳米颗粒（AuNPs）和鲁米诺的双功能还原氧化石墨烯（rGO）杂化物（rGO / AuNP / luminol）。制备的rGO / AuNP /鲁米诺杂化物与H ₂ O ₂混合后，表现出增强的和持久的化学发光（CL）性能，并且在1小时内没有衰减。这是由于OH产生，O- ₂ ˙ ^- ，¹ Ò ₂，和π-CC˙种。开发了基于rGO / AuNP / luminol混合CL增强系统的灵敏且选择性的CL传感器，用于在0.71至11.43μM的线性范围内进行胆固醇定量，检测极限为0.55μM。此外，所提出的方法已成功地用于测定人血清样品中的胆固醇。