We report the first amperometric method for the simultaneous determination of tert-butylhydroquinone (tBHQ), propyl gallate (PG), and butylated hydroxyanisole (BHA) using flow injection analysis coupled to multiple-pulse amperometry. A sequence of potential pulses was selected in order to detect tBHQ, PG, and BHA separately in a single injection step at a glassy carbon electrode without the need of a preliminary separation. A mixture of methanol and 0.040 M Britton-Robinson buffer was used both as a carrier solution and for dilution of analyzed solutions before injection. The method is precise (RSD < 5%, n = 10), fast (a frequency of 140 injections h⁻¹), provides sufficiently low quantification limits (2.51, 1.45, and 0.85 μmol L^–1 for tBHQ, PG, and BHA, respectively) and can be easily applied without high demands on instrumentation. As a practical application, the determination of these antioxidants contained in commercial chewing gum samples was carried out by applying a simple extraction procedure.

我们报告了使用流动注射分析和多脉冲安培法同时测定叔丁基对苯二酚（tBHQ），没食子酸丙酯（PG）和丁基化羟基茴香醚（BHA）的第一种安培法。选择了一系列潜在脉冲，以便在单个碳纤维电极上的单个进样步骤中分别检测tBHQ，PG和BHA，而无需进行初步分离。甲醇和0.040 M Britton-Robinson缓冲液的混合物既用作载液，又用于进样前稀释分析液。该方法精密度高（RSD <5％，n = 10），快速（140次进样h ^-1的频率），提供了足够低的定量限（2.51、1.45和0.85μmolL^–1分别用于tBHQ，PG和BHA，并且可以很容易地应用而对仪器的要求不高。在实际应用中，通过应用简单的提取程序即可测定市售口香糖样品中所含的这些抗氧化剂。