Dengue is a RNA viral illness of the genus Flavivirus which can cause, depending on the pervasiveness of the infection, hemorrhagic dengue fever or dengue shock syndrome. Herein we present an electrochemical label free approach enabling the rapid sensitive quantification of NS1 and IgG (supporting an ability to distinguish primary and secondary infections). Using a bifunctional SAM containing PEG moieties and a tethered redox thiol, both markers are detectable across clinically relevant levels by label free impedance derived redox capacitance. A subsequent frequency specific immittance function approach enables assaying (within seconds) with no impairment of analytical quality (linearity, sensitivity and variance).

登革热是黄病毒属的RNA病毒性疾病，根据感染的普遍程度，可能导致出血性登革热或登革热休克综合症。在本文中，我们提出了一种无电化学标记的方法，可以对NS1和IgG进行快速灵敏的定量（支持区分原发性和继发性感染的能力）。使用包含PEG部分和束缚的氧化还原硫醇的双功能SAM，可通过无标记阻抗衍生的氧化还原电容跨临床相关水平检测两种标记。随后的频率特定的阻抗函数方法可以在几秒钟内进行分析，而不会损害分析质量（线性，灵敏度和方差）。