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Renal Clearable New NIR Probe: Precise Quantification of Albumin in Biofluids and Fatty Liver Disease State Identification through Tissue Specific High Contrast Imaging in Vivo
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-09-12 00:00:00 , DOI: 10.1021/acs.analchem.7b02187
Gourab Dey 1 , Vikas Singh 2 , Jayant Dewangan 3 , P. Vineeth Daniel 1 , Mohan Kamthan 4 , Debabrata Ghosh 2 , Prosenjit Mondal 1 , Subrata Ghosh 1
Affiliation  

Development of a highly photostable, renal clearable, and nontoxic new NIR probe (CyG) for precise quantification of albumin in different biofluids and liver targeted in vivo albumin visualization is demonstrated. CyG’s inherent property to interact selectively with albumin among different biomolecules in intracellular environment with high degree of sensitivity helps CyG in targeted liver imaging. In addition to its long excitation/emission wavelengths (λex = 740 nm, λem = 804 nm), which are much above the biological tissue opaque window (400–700 nm) ensuring better photon penetration, diminished tissue autofluorescence and high contrasts, its molecular mass and size are far below the renal cutoff and hence, CyG qualifies as imaging material for clinical studies. We anticipate that CyG will provide new strategies to overcome the pitfall of present day albumin detection methods as well as accelerate the detection process at relatively lower costs without compromising the accuracy of detection. Moreover, the renal excretion kinetic and intrahepatic albumin binding affinity of CyG can further be used to differentiate between fatty liver from healthy liver in an experimentally arrived mouse model using noninvasive technique.

中文翻译:

肾脏可清除的新型NIR探针:生物体中白蛋白的精确定量和通过体内组织特异性高对比度成像的脂肪肝疾病状态识别

展示了开发出一种高度光稳定,可肾脏清除且无毒的新型NIR探针(CyG),用于精确定量不同生物流体中的白蛋白以及肝脏靶向的体内白蛋白可视化。CyG具有与细胞内环境中不同生物分子之间的白蛋白选择性相互作用的固有属性,具有高度的敏感性,可帮助CyG进行有针对性的肝脏成像。除了其长的激发/发射波长(λex = 740 nm,λem= 804 nm),远远高于生物组织的不透明窗口(400–700 nm),可确保更好的光子穿透,组织自发荧光减弱和高对比度,其分子质量和大小远低于肾脏截止值,因此CyG符合成像的资格用于临床研究的材料。我们预计CyG将提供新的策略来克服当今白蛋白检测方法的缺陷,并以相对较低的成本加速检测过程,而不会影响检测的准确性。此外,CyG的肾脏排泄动力学和肝内白蛋白结合亲和力可进一步用于通过无创技术在实验到达的小鼠模型中区分脂肪肝与健康肝。
更新日期:2017-09-12
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