当前位置：
X-MOL 学术
›
Anal. Chem.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Excimer Emission-Based Fluorescent Probe Targeting Caspase-3
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-09-12 00:00:00 , DOI: 10.1021/acs.analchem.7b02790 Tae-Il Kim 1 , Hanyong Jin 2 , Jeehyeon Bae 2 , Youngmi Kim 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-09-12 00:00:00 , DOI: 10.1021/acs.analchem.7b02790 Tae-Il Kim 1 , Hanyong Jin 2 , Jeehyeon Bae 2 , Youngmi Kim 1
Affiliation
|
A fluorescent probe based on an excimer-forming benzothiazolyl-cyanovinylene (CV) dye was developed to target the apoptotic protease caspase-3. Upon the action of caspase-3, the water-soluble fluorescent probe Ac-DEVD-NH-CV, which is weakly green emissive in aqueous solution, is converted to hydrophobic CV-NH2, which spontaneously aggregates. Aggregation of CV-NH2 promotes excimer emission of the CV dye, which allows for the study of caspase-3 activity in vitro and for imaging the activity of the enzyme in living cells because of the large red shift and enhanced fluorescence signal of the probe.
中文翻译：
靶向Caspase-3的基于准分子发射的荧光探针
基于形成准分子的苯并噻唑基-氰基亚乙烯基（CV）染料的荧光探针被开发为靶向凋亡蛋白酶caspase-3。在caspase-3的作用下，在水溶液中呈绿色弱发射的水溶性荧光探针Ac-DEVD- NH-CV转化为疏水性CV-NH 2，后者自发聚集。CV-NH 2的聚集促进了CV染料的受激准分子发射，由于探针的大红移和增强的荧光信号，它使得可以在体外研究caspase-3的活性并可以对活细胞中酶的活性进行成像。 。
更新日期：2017-09-12
中文翻译：
靶向Caspase-3的基于准分子发射的荧光探针
基于形成准分子的苯并噻唑基-氰基亚乙烯基（CV）染料的荧光探针被开发为靶向凋亡蛋白酶caspase-3。在caspase-3的作用下，在水溶液中呈绿色弱发射的水溶性荧光探针Ac-DEVD- NH-CV转化为疏水性CV-NH 2，后者自发聚集。CV-NH 2的聚集促进了CV染料的受激准分子发射，由于探针的大红移和增强的荧光信号，它使得可以在体外研究caspase-3的活性并可以对活细胞中酶的活性进行成像。 。
京公网安备 11010802027423号