当前位置：
X-MOL 学术
›
Anal. Chem.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Identification and Quantitation of C═C Location Isomers of Unsaturated Fatty Acids by Epoxidation Reaction and Tandem Mass Spectrometry
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-09-12 00:00:00 , DOI: 10.1021/acs.analchem.7b01870 Yaoyao Zhao 1 , Hansen Zhao 2 , Xu Zhao 2 , Jia Jia 2 , Qiang Ma 3 , Sichun Zhang 2 , Xinrong Zhang 2 , Hitoshi Chiba 1 , Shu-Ping Hui 1 , Xiaoxiao Ma 4
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-09-12 00:00:00 , DOI: 10.1021/acs.analchem.7b01870 Yaoyao Zhao 1 , Hansen Zhao 2 , Xu Zhao 2 , Jia Jia 2 , Qiang Ma 3 , Sichun Zhang 2 , Xinrong Zhang 2 , Hitoshi Chiba 1 , Shu-Ping Hui 1 , Xiaoxiao Ma 4
Affiliation
|
Unsaturated fatty acids (FAs) serve as nutrients, energy sources, and signaling molecules for organisms, which are the major components for a large variety of lipids. However, structural characterization and quantitation of unsaturated FAs by mass spectrometry remain an analytical challenge. Here, we report the coupling of epoxidation reaction of the C═C in unsaturated FAs and tandem mass spectrometry (MS) for rapid and accurate identification and quantitation of C═C isomers of FAs in a shotgun lipidomics approach. Epoxidation of the C═C leads to the production of an epoxide which, upon collision induced dissociation (CID), produces abundant diagnostic ions indicative of the C═C location. The total intensity of the same set of diagnostic ions for one specific FA C═C isomer was also used for its relative and absolute quantitation. The simple experimental setup, rapid reaction kinetics (<2 min), high reaction yield (>90% for monounsaturated FAs), and easy-to-interpret tandem MS spectra enable a promising methodology particularly for the analysis of unsaturated FAs in complex biological samples such as human plasma and animal tissues.
中文翻译：
环氧化反应-串联质谱法鉴定和定量不饱和脂肪酸的C═C位置异构体
不饱和脂肪酸（FAs）可作为有机物的营养物质，能源和信号分子，而有机物是多种脂质的主要成分。然而，通过质谱对不饱和脂肪酸的结构表征和定量分析仍然是一个分析难题。在这里，我们报告不饱和脂肪酸中C lipidC的环氧化反应与串联质谱法（MS）的耦合，以便在a弹枪脂质组学方法中快速准确地鉴定和定量FAs的C═C异构体。C═C的环氧化会导致生成环氧化合物，在碰撞诱导解离（CID）后，会生成大量指示C diagnosticC位置的诊断离子。对于一个特定的FAC═C异构体，同一组诊断离子的总强度也用于其相对和绝对定量。
更新日期：2017-09-12
中文翻译：
环氧化反应-串联质谱法鉴定和定量不饱和脂肪酸的C═C位置异构体
不饱和脂肪酸（FAs）可作为有机物的营养物质，能源和信号分子，而有机物是多种脂质的主要成分。然而，通过质谱对不饱和脂肪酸的结构表征和定量分析仍然是一个分析难题。在这里，我们报告不饱和脂肪酸中C lipidC的环氧化反应与串联质谱法（MS）的耦合，以便在a弹枪脂质组学方法中快速准确地鉴定和定量FAs的C═C异构体。C═C的环氧化会导致生成环氧化合物，在碰撞诱导解离（CID）后，会生成大量指示C diagnosticC位置的诊断离子。对于一个特定的FAC═C异构体，同一组诊断离子的总强度也用于其相对和绝对定量。
京公网安备 11010802027423号