A Low Energy Route to DNA-Wrapped Carbon Nanotubes via Replacement of Bile Salt Surfactants,Analytical Chemistry

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A Low Energy Route to DNA-Wrapped Carbon Nanotubes via Replacement of Bile Salt Surfactants
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-09-11 00:00:00 , DOI: 10.1021/acs.analchem.7b02637
Jason K. Streit ₁ , Jeffrey A. Fagan ₁ , Ming Zheng ₁

Affiliation

DNA-wrapped carbon nanotubes are a class of bionano hybrid molecules that have enabled carbon nanotube sorting, controlled assembly, and biosensing and bioimaging applications. The current method of synthesizing these hybrids via direct sonication of DNA/nanotube mixtures is time-consuming and not suitable for high-throughput synthesis and combinatorial sequence screening. Additionally, the direct sonication method does not make use of nanotubes presorted by extensively developed surfactant-based methods, is not effective for large diameter (>1 nm) tubes, and cannot maintain secondary and tertiary structural and functional domains present in certain DNA sequences. Here, we report a simple, quick, and robust process to produce DNA-wrapped carbon nanotube hybrids with nanotubes of broad diameter range and DNA of arbitrary sequence. This is accomplished by exchanging strong binding bile salt surfactant coating with DNA in methanol/water mixed solvent and subsequent precipitation with isopropyl alcohol. The exchange process can be completed within 10 min and converts over 90% nanotubes into the DNA wrapped form. Applying the exchange process to nanotubes presorted by surfactant-based methods, we show that the resulting DNA-wrapped carbon nanotubes can be further sorted to produce nanotubes with defined handedness, helicity, and endohedral filling. The exchange method greatly expands the structural and functional variety of DNA-wrapped carbon nanotubes and opens possibilities for DNA-directed assembly of structurally sorted nanotubes and high-throughput screening of properties that are controlled by the wrapping DNA sequences.

中文翻译：

通过替换胆汁盐表面活性剂的低能量路线，以DNA包裹碳纳米管

DNA包裹的碳纳米管是一类可实现碳纳米管分选，可控组装以及生物传感和生物成像应用的生物纳米杂化分子。通过直接超声处理DNA /纳米管混合物来合成这些杂种的当前方法是耗时的，并且不适用于高通量合成和组合序列筛选。另外，直接超声处理方法不利用通过广泛开发的基于表面活性剂的方法预先分选的纳米管，对大直径（> 1 nm）的管无效，并且不能维持某些DNA序列中存在的二级和三级结构和功能域。在这里，我们报告了一种简单，快速且可靠的方法来生产具有宽直径范围的纳米管和任意序列的DNA的DNA包裹的碳纳米管杂化物。这是通过在甲醇/水混合溶剂中用DNA交换结合力强的胆盐表面活性剂涂层并随后用异丙醇沉淀来实现的。交换过程可以在10分钟内完成，并将超过90％的纳米管转化为DNA包裹的形式。将交换过程应用于通过基于表面活性剂的方法进行预分选的纳米管，我们表明所得的DNA包裹的碳纳米管可以进一步分选，以产生具有确定的手性，螺旋度和内面填充的纳米管。这种交换方法极大地扩展了DNA包裹的碳纳米管的结构和功能多样性，并为结构分类的纳米管的DNA定向组装和通过包裹DNA序列控制的特性的高通量筛选提供了可能性。

更新日期：2017-09-12

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