Biomolecular corona formation: nature and bactericidal impact on surface-modified silica nanoparticles,Journal of Materials Chemistry B

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Biomolecular corona formation: nature and bactericidal impact on surface-modified silica nanoparticles
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2017-08-22 00:00:00 , DOI: 10.1039/c7tb01744h
Maiara Emer _{1,

2,

3,

4,

5} , Mateus Borba Cardoso _{1,

2,

3,

4,

5}

Affiliation

Biological fluids contain a plethora of different components that can coat nanoparticle's surface and critically affect their interaction with living systems. The recent literature has focused efforts on understanding the overall protein role in this nanoparticle's coating (commonly called a protein corona) without considering other components’ influence. Here, we report a protocol to study the relationship between biological fluid components excluding proteins (referred to as a biomolecular corona) and nanoparticles. For this purpose, functionalized silica nanoparticles were duly synthesized to experimentally investigate the extent of biomolecular corona adsorption over different surfaces. This adsorption was directly correlated with the silica surface chemical constitution, while the extent of biomolecular corona coating affected the overall surface charge and the colloidal stability of the functionalized nanoparticles. In addition, the bactericidal properties of biomolecular corona-coated nanoparticles were tested against two medically relevant bacteria (Escherichia coli and Staphylococcus aureus). Biological results indicated that the presence and the extent of the biomolecular corona affect the bactericidal properties of the nanoparticles.

中文翻译：

生物分子电晕的形成：性质和对表面改性二氧化硅纳米粒子的杀菌作用

生物流体包含过多的不同成分，可以覆盖纳米粒子的表面并严重影响其与生物系统的相互作用。最近的文献集中精力在不考虑其他成分影响的情况下，了解该纳米颗粒涂层（通常称为蛋白质电晕）中的整体蛋白质作用。在这里，我们报告了一项协议，以研究除蛋白质（称为生物分子电晕）以外的生物流体成分与纳米颗粒之间的关系。为此目的，适当地合成了官能化的二氧化硅纳米粒子，以实验研究生物分子电晕在不同表面上的吸附程度。这种吸附与二氧化硅表面化学成分直接相关，而生物分子电晕涂层的程度会影响整体表面电荷和功能化纳米粒子的胶体稳定性。此外，还测试了生物分子电晕涂层纳米颗粒对两种医学上相关细菌的杀菌性能（大肠杆菌和金黄色葡萄球菌）。生物学结果表明，生物分子电晕的存在和程度影响纳米颗粒的杀菌性能。

更新日期：2017-09-12

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