Engineering an efficient biomolecular circuit often requires time-consuming iterations of optimization. Cell-free protein expression systems allow rapid testing of biocircuits in vitro, speeding the design–build–test cycle of synthetic biology. In this paper, we combine this with droplet microfluidics to densely scan a transcription–translation biocircuit space. Our system assays millions of parameter combinations per hour, providing a detailed map of function. The ability to comprehensively map biocircuit parameter spaces allows accurate modeling to predict circuit function and identify optimal circuits and conditions.

中文翻译：

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基于无细胞提取物的微滴微流体优化生物分子回路

设计高效的生物分子电路通常需要耗时的优化迭代。无细胞蛋白质表达系统可以在体外快速测试生物电路，从而加快合成生物学的设计，构建和测试周期。在本文中，我们将其与液滴微流体相结合以密集扫描转录-翻译生物回路空间。我们的系统每小时分析数百万个参数组合，提供详细的功能图。全面映射生物电路参数空间的能力允许进行精确建模，以预测电路功能并确定最佳电路和条件。