Paeonol, an active constituent in the root bark of Paeonia suffruticosa Andrews, is used to treat inflammation, headache and other diseases in clinic. Though the data on pharmacological researches of paeonol abounds, its metabolic profile is not so clear. It is essential to systematically characterize the in vivo metabolites in order to better understand its mechanism of action. In this study, ultra performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UPLC-ESI-Q/TOF-MS) with an integrative strategy was developed for analysis of paeonol metabolites. As a result, based on seven reference substances isolated or synthesized, twenty-five metabolites were detected and identified in urine, feces, bile and plasma of rats after oral administration of paeonol. To the best of our knowledge, 14 of these metabolites have not been reported previously. In addition, the dominating metabolic fates were oxidation, demethylation, hydrogenation, glucuronic acid and sulfate conjugations, and hydrogenation of paeonol was reported for the first time. This research provides scientific and reliable support for full understanding of the metabolic profiling of paeonol.

eon药是白e药根皮中的活性成分，在临床上用于治疗炎症，头痛和其他疾病。尽管有关丹皮酚的药理研究的数据很多，但其代谢特征尚不十分清楚。系统地表征体内特性至关重要代谢物，以便更好地了解其作用机理。在这项研究中，开发了一种具有综合策略的超高效液相色谱结合电喷雾电离四极杆飞行时间串联质谱（UPLC-ESI-Q / TOF-MS），用于分析丹皮酚的代谢物。结果，基于分离或合成的七种参考物质，口服丹皮酚后，在大鼠的尿液，粪便，胆汁和血浆中检测并鉴定出25种代谢物。据我们所知，这些代谢物中的14种尚未被报道过。此外，主要的代谢命运是氧化，脱甲基，氢化，葡萄糖醛酸和硫酸盐结合，并且首次报道了丹皮酚的氢化。