Alzheimer’s disease (AD) biomarkers can reflect the neurochemical indicators used to estimate the risk in clinical nephrology. Apolipoprotein E (ApoE) is an early biomarker for AD in clinical diagnosis. In this research, through bactrian camel immunization, lymphocyte isolation, RNA extraction, and library construction, ApoE-specific Nbs with high affinity were successfully separated from an immune phage display nanobody library. Herein, a colorimetric immunosensor was developed for the point-of-care testing of ApoE by layer-by-layer nanoassembly techniques and novel nanobodies (Nbs). Using highly oriented Nbs as the capture and detection antibodies, an on-site immunosensor was developed by detecting the mean gray value of fade color due to the glutaraldehyde@3-aminopropyltrimethoxysilane oxidation by H₂O₂. The detection limit of AopE is 0.42 pg/mL, and the clinical analysis achieves a good performance. The novel easily operated immunosensor may have potential application in the clinical diagnosis and real-time monitoring for AD.

中文翻译：

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用于护理点测试的基于纳米抗体的载脂蛋白E免疫传感器

阿尔茨海默氏病（AD）生物标志物可以反映用于评估临床肾脏病风险的神经化学指标。载脂蛋白E（ApoE）是临床诊断AD的早期生物标记。在这项研究中，通过双峰驼免疫，淋巴细胞分离，RNA提取和文库构建，从免疫噬菌体展示纳米抗体库中成功分离出具有高亲和力的ApoE特异性Nb。本文中，开发了一种比色免疫传感器，用于通过逐层纳米组装技术和新型纳米抗体（Nbs）对ApoE进行即时检测。使用高度定向的Nbs作为捕获和检测抗体，通过检测由于戊二醛@ 3-氨基丙基三甲氧基硅烷被H ₂ O氧化而引起的褪色平均灰度值，开发了一种现场免疫传感器。₂。AopE的检出限为0.42 pg / mL，临床分析取得了良好的性能。新型易于操作的免疫传感器在AD的临床诊断和实时监测中可能具有潜在的应用。