By manipulating the reaction conditions, furandicarboxylic acid (FDCA) was prepared by the biooxidation of hydroxymethyl furfural (HMF) in a continuous one-pot reaction using galactose oxidase M_3–5, periplasmic aldehyde oxidase (PaoABC), catalase and horseradish peroxidase. In addition, PaoABC was successfully entrapped in a SiO₂ hydrogel and recycled 14 times without loss of activity. The catalyst was able to tolerate up to 200 mM DFF concentration giving FDCA in full conversion with very promising TOF and TON values.

中文翻译：

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HMF连续氧化为FDCA以及周质醛氧化酶（PaoABC）的固定和稳定

通过控制反应条件，使用半乳糖氧化酶M _3-5，周质醛氧化酶（PaoABC），过氧化氢酶和辣根过氧化物酶，通过连续一锅法对羟甲基糠醛（HMF）进行生物氧化，制备了呋喃二甲酸（FDCA）。此外，PaoABC被成功地包裹在SiO ₂水凝胶中，并循环使用了14次而没有失去活性。该催化剂能够耐受高达200 mM的DFF浓度，从而使FDCA完全转化，并具有非常有希望的TOF和TON值。